Effect of propofol on myelin basic protein expression and myelination of oligodendrocytes in neonatal SD rats.
10.12122/j.issn.1673-4254.2019.08.12
- Author:
Xin ZHANG
1
;
Chunshui LIN
1
;
Peipei GUO
1
;
Jun QIN
2
;
Xiuxiu QIN
1
;
Weidong LIANG
1
Author Information
1. Department of Anesthesiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
2. Department of Anesthesiology, Longgang Orthopedics Hospital, Shenzhen 518116, China.
- Publication Type:Journal Article
- Keywords:
myelin basic protein;
myelin sheath;
neonates;
oligodendrocytes;
propofol
- MeSH:
Animals;
Myelin Basic Protein;
Oligodendroglia;
Propofol;
RNA, Messenger;
Rats;
Rats, Sprague-Dawley
- From:
Journal of Southern Medical University
2019;39(8):950-956
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of different doses of propofol on myelin basic protein (MBP) synthesis and myelination of oligodendrocytes in neonatal SD rats.
METHODS:A total of 57 neonatal SD rats (7 days old) were randomly divided into control group (=13), vehicle (fat emulsion) group (=5), and 25, 50 and 100 mg/kg propofol groups (=13 in each group). Eight hours after a single intraperitoneal injection of propofol or the vehicle, the rats were examined for expressions of mRNA, caspase-3 mRNA, cleaved caspase-3 and MBP in the brain tissues using qPCR and Western blotting. Immunofluorescence assay was used to detect the apoptosis of the oligodendrocytes at 8 h after the injection and the myelination of the corpus callosum and internal capsule at 24 h.
RESULTS:Compared with the control group, the neonatal rats with propofol injections showed significantly down-regulated expressions of mRNA and MBP protein in the brain tissue ( < 0.05). Propofol dose-dependently increased the transcription level of caspase-3 and the protein levels of cleaved caspase-3 at 8 h after the injection ( < 0.05). Propofol injection significantly increased the apoptosis of the oligodendrocytes, and the effect was significantly stronger in 50 and 100 mg/kg groups than in 25 mg/kg group ( < 0.05). At 24 h after propofol injection, myelin formation was significantly decreased in the corpus callosum of the neonatal rats in 100 mg/kg propofol group and in the internal capsule in 50 and 100 mg/kg groups ( < 0.05).
CONCLUSIONS:In neonatal SD rats, propofol can dose-dependently promote oligodendrocyte apoptosis, decrease MBP expressions in the brain, and suppress myelin formation in the corpus callosum and the internal capsule.
