Expressions of HSP110 family members in the testes and epididymis of mice at different stages of development and their regulation by hormones.
10.12122/j.issn.1673-4254.2019.09.13
- Author:
Chengting RONG
1
;
Ziwei DU
1
;
Juan LIU
2
;
Xinan WU
1
Author Information
1. Department of Pharmacy, Hefei BOE Hospital, Anhui, Hefei 230041, China.
2. Central Laboratory of Yantai Yuhuangding Hospital, Yantai 264000, China.
- Publication Type:Journal Article
- Keywords:
HSP110 family;
apoptosis;
development;
hormone regulation
- MeSH:
Animals;
Epididymis;
growth & development;
Gene Expression Regulation, Developmental;
HSP110 Heat-Shock Proteins;
genetics;
metabolism;
Male;
Mice;
Orchiectomy;
Testis;
growth & development;
Testosterone;
pharmacology
- From:
Journal of Southern Medical University
2019;39(9):1083-1088
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the expressions of the members of HSP110 family in the testis and epididymis of mice at different stages of development and whether they are regulated by hormones.
METHODS:The testicular and epididymis tissues of mice at different ages (14, 21, 28, 35, 42, 49, 70, and 90 days after birth, 3 mice at each age) were collected for RT-PCR detection of the expression levels of HSP110 family members. Forty-eight mice were randomized into 3 groups for sham operation, castration, or castration with testosterone injections every other day (starting at 7 days after castration), and at 1, 3, 5, and 7 days after first testosterone injection, the expressions of HSP110 family in the epididymis were detected using RT-PCR.
RESULTS:The mRNA expression levels of HSP110 family members underwent obvious variations with the development of the mice: , and expressions in the testicles of the mice first increased and then decreased, and gradually became stable; they also exhibited similar temporal patterns of changes in the epididymis. In the castrated mice, the mRNA expressions of and in the epididymis decreased significantly with the reduction of serum hormone levels ( < 0.05), and became normal after the supplementation of exogenous hormone.
CONCLUSIONS:The expression levels of HSP110 family are affected by developmental regulation, and the expressions of and are under the regulation by hormones.
