Effect of -induced changes in tumor tissue acoustic properties on efficacy of high-intensity focused ultrasound ablation.
10.12122/j.issn.1673-4254.2019.09.02
- Author:
Die XU
1
;
Yong LUO
1
;
Xuan GAO
1
;
Jie XIONG
1
;
Binglei JIANG
1
;
Yaotai WANG
1
;
Yu TANG
1
;
Fujie JIANG
1
;
Lu WANG
1
;
Huanan LI
1
;
Hai QIAO
1
;
Jianzhong ZOU
1
Author Information
1. State Key Laboratory of Ultrasound Engineering in Medicine Co-Founded by Chongqing and the Ministry of Science and Technology, College of Biomedical Engineering, Chongqing Key Laboratory of Biomedical Engineering, Chongqing Collaborative Innovation Center for Minimally-invasive and Noninvasive Medicine, Chongqing Medical University, Chongqing 400016, China.
- Publication Type:Journal Article
- Keywords:
Bifidobacterium;
acoustic characteristics;
high-intensity focused ultrasound;
tumor
- MeSH:
Acoustics;
Animals;
Bifidobacterium;
pathogenicity;
Breast Neoplasms;
pathology;
Collagen;
Elasticity Imaging Techniques;
High-Intensity Focused Ultrasound Ablation;
Humans;
Mice;
Mice, Nude;
Neoplasm Transplantation;
Random Allocation
- From:
Journal of Southern Medical University
2019;39(9):1009-1016
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of on the acoustic characteristics of tumor tissue and how such acoustic changes affect the efficacy of high-intensity focused ultrasound (HIFU) ablation in nude mice.
METHODS:Forty mice bearing human breast cancer cell (MDA-MB-231) xenograft were randomized into experimental group (=20) and control group (=20) for intravenous injection of suspension (200 μL, 4 × 10 cfu/mL) and PBS (200 μL) for 3 consecutive days, respectively. Before and at 3 and 7 days after the first injection, shear wave elastography was used to evaluate the hardness of the tumor tissue. On day 7 after the first injection, 10 mice from each group were sacrificed and the sound velocity and sound attenuation of the tumor tissues were measured. The changes in the collagen fibers in the tumors were evaluated using Masson staining, and neovascularization in the tumor was assessed with immunohistochemistry for platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31). The remaining 10 tumor-bearing mice in each group were subjected to HIFU ablation, and the ablation efficiency was evaluated by assessing the changes in irradiation gray values, coagulative necrosis volume, energy efficiency factor (EEF) and irradiation area and by pathological examination with HE staining.
RESULTS:In the experimental group, the collagen fibers in the tumor tissues were strong and densely aligned, and the tumors contained fewer new blood vessels showing strip-or spot-like morphologies. In the control group, the collagen fibers in the tumors were thin and loosely arranged, and the tumors showed abundant elongated or round new blood vessels. colonized in the tumor 7 days after the injection, and the tumor hardness was significantly greater in the experimental group than in the control group (=0.01); the acoustic velocity (=0.001) and the acoustic attenuation (=0.000) of the tumor tissues were also greater in the experimental group. HIFU irradiation resulted in significantly greater changes in the gray scale of tumor (=0.0006) and larger coagulative necrosis volume (=0.0045) in the experimental group than in the control group, and the EEF was significantly smaller in the experimental group (=0.0134).
CONCLUSIONS: can cause changes in collagen fiber content, acoustic velocity and attenuation in the tumor tissue and reduce the EEF of HIFU irradiation, thereby improving the efficacy of HIFU irradiation.
