Effect of cinobufagin on transient outward potassium current in dorsal root ganglion cells of rats with cancer-induced bone pain.
10.12122/j.issn.1673-4254.2019.09.12
- Author:
Shiyu ZHU
1
;
Dan LIU
1
;
Wei HU
1
;
Hongwei YANG
1
Author Information
1. Medical College of China Three Gorges University, Yichang 443002, China.
- Publication Type:Journal Article
- Keywords:
analgesic effect;
cancer-induced bone pain;
cinobufagin;
dorsal root ganglion;
transient outward potassium current
- MeSH:
Analgesics;
pharmacology;
Animals;
Bufanolides;
pharmacology;
Cancer Pain;
drug therapy;
Cells, Cultured;
Ganglia, Spinal;
drug effects;
Patch-Clamp Techniques;
Potassium Channels;
metabolism;
Rats;
Rats, Sprague-Dawley
- From:
Journal of Southern Medical University
2019;39(9):1078-1082
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the effect of cinobufagin on transient outward potassium current () in rat dorsal root ganglion cells of cancer-induced bone pain (CIBP) and explore the possible analgesic mechanism of cinobufagin.
METHODS:Whole cell patch clamp technique was used to examine the effect of cionbufagin on in acutely isolated dorsal root ganglion (DRG) cells from normal SD rats and rats with bone cancer pain.
RESULTS:The DRG cells from rats with CIBP showed obviously decreased current density, an activation curve shift to the right, and an inactivation curve shift to the left. Cinobufagin treatment significantly increased the current density and reversed the changes in the activation and inactivation curves in the DRG cells.
CONCLUSIONS: current is decreased in DRG neurons from rats with CIBP. Cinobufagin can regulate the activation and inactivation of current in the DRG cells, which may be related to its analgesic mechanism.
