Protective effect of against oxidative stress in neonatal mice with necrotizing enterocolitis.
10.12122/j.issn.1673-4254.2019.10.14
- Author:
Jia TANG
1
;
Chunbao GUO
2
;
Fang GONG
1
Author Information
1. Department of Pediatrics, Yongchuan Hospital Affiliated to Chongqing Medical University, Chongqing 402160, China.
2. Department of Hepatobiliary Surgery, Children's Hospital Affiliated to Chongqing Medical University, Chongqing 400016, China.
- Publication Type:Journal Article
- Keywords:
Lactobacillus reuteri DSM17938;
inflammatory cytokines;
necrotizing enterocolitis;
oxidative stress
- From:
Journal of Southern Medical University
2019;39(10):1221-1226
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the protective effect of DSM17938 strain against oxidative stress in a neonatal mouse model of necrotizing enterocolitis (NEC) and explore the possible mechanism.
METHODS:Ninety-six 10-day-old neonatal C57BL/6J mice were equally randomized into control group, NEC group, and NEC+ group. The pathological changes of the ileocecal intestinal tissue were evaluated with HE staining and double-blind pathological scoring. The mRNA and protein expressions of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the intestinal tissues were detected using quantitative real-time PCR and ELISA, respectively. Colorimetric assays were used to determine the activity of superoxide dismutase (SOD) and its inhibition rate, malondialdehyde (MDA), glutathione (GSH), oxidized glutathione (GSSG), and GSSG/ GSH ratio.
RESULTS:Compared with those in the control group, the neonatal mice in NEC group showed significant weight loss ( < 0.05), obvious intestinal injury, increased pathological scores ( < 0.05), increased expressions of TNF-α and IL-1β mRNA and proteins ( < 0.05), decreased SOD activity and inhibition rate, decreased GSH, and significantly increased MDA, GSSG, and GSSG/GSH ratios ( < 0.05). Treatment with obviously decreased the pathological scores, expressions of TNF-α and IL-1β ( < 0.05), MDA, GSSG, and GSSG/GSH ratio ( < 0.05), and significantly increased SOD activity, its inhibition rate, and GSH level in the mice with NEC, but the survival rate was not significantly different between NEC and -treated groups ( > 0.05).
CONCLUSIONS: DSM17938 can offer protection against NEC in mice by reducing oxidative stress and increasing antioxidant capacity of the intestinal tissue to suppress intestinal inflammations.
