Antioxidant and Anti-inflammatory Capacity of Ferulic Acid Released from Wheat Bran by Solid-state Fermentation of Aspergillus niger.
- Author:
Zhi Na YIN
1
;
Wen Jia WU
2
;
Chong Zhen SUN
3
;
Hui Fan LIU
3
;
Wen Bo CHEN
3
;
Qi Ping ZHAN
3
;
Zhuo Gui LEI
3
;
Xuan XIN
3
;
Juan Juan MA
3
;
Kun YAO
3
;
Tian MIN
3
;
Meng Meng ZHANG
3
;
Hui WU
3
Author Information
- Publication Type:Journal Article
- Keywords: Anti-inflammatory activity; Antioxidant activity; Fermentation; Ferulic acid; Fungal identification; Oxidative hemolysis
- MeSH: Animals; Anti-Inflammatory Agents; metabolism; pharmacology; Antioxidants; metabolism; pharmacology; Aspergillus niger; genetics; isolation & purification; metabolism; Coumaric Acids; metabolism; pharmacology; DNA, Fungal; analysis; Dietary Fiber; microbiology; Erythrocytes; drug effects; metabolism; Fermentation; Hep G2 Cells; Humans; Interleukin-6; metabolism; Lipopolysaccharides; pharmacology; Mice; RAW 264.7 Cells; Sheep; Tumor Necrosis Factor-alpha; metabolism
- From: Biomedical and Environmental Sciences 2019;32(1):11-21
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:A strain of Aspergillus niger (A. niger), capable of releasing bound phenolic acids from wheat bran, was isolated. This strain was identified by gene sequence identification. The antioxidant and anti-inflammatory capacity of ferulic acid released from wheat bran by this A. niger strain (FA-WB) were evaluated.
METHODS:Molecular identification techniques based on PCR analysis of specific genomic sequences were conducted; antioxidant ability was examined using oxygen radical absorbance capacity (ORAC), cellular antioxidant activity (CAA) assays, and erythrocyte hemolysis assays. RAW264.7 cells were used as a model to detect anti-inflammatory activity.
RESULTS:The filamentous fungal isolate was identified to be A. niger. ORAC and CAA assay showed that FA-WB had better antioxidant activity than that of the ferulic acid standard. The erythrocyte hemolysis assay results suggested that FA-WB could attenuate AAPH-induced oxidative stress through inhibition of reactive oxy gen species (ROS) generation. FA-WB could significantly restore the AAPH-induced increase in intracellular antioxidant enzyme activities to normal levels as well as inhibit the intracellular malondialdehyde formation. TNF-a, IL-6, and NO levels indicated that FA-WB can inhibit the inflammation induced by lipopolysaccharide (LPS).
CONCLUSION:Ferulic acid released from wheat bran by a new strain of A. niger had good anti-inflammatory activity and better antioxidant ability than standard ferulic acid.