Antioxidant and Anti-inflammatory Capacity of Ferulic Acid Released from Wheat Bran by Solid-state Fermentation of Aspergillus niger.

Zhi Na Yin; Wen Jia WU; Chong Zhen Sun; Hui Fan Liu; Wen Bo Chen; Qi Ping Zhan; Zhuo Gui Lei; Xuan Xin; Juan Juan MA; Kun Yao; Tian Min; Meng Meng Zhang; Hui WU

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Antioxidant and Anti-inflammatory Capacity of Ferulic Acid Released from Wheat Bran by Solid-state Fermentation of Aspergillus niger.

Author: Zhi Na YIN ¹ ; Wen Jia WU ² ; Chong Zhen SUN ³ ; Hui Fan LIU ³ ; Wen Bo CHEN ³ ; Qi Ping ZHAN ³ ; Zhuo Gui LEI ³ ; Xuan XIN ³ ; Juan Juan MA ³ ; Kun YAO ³ ; Tian MIN ³ ; Meng Meng ZHANG ³ ; Hui WU ³
Author Information

1. Biotechnology, guangdong polytechnic science and trade, Guangzhou 510640, Guangdong, China.
2. School of Medical Technology and Nursing, Shenzhen Polytechnic, Shenzhen 518000, Guangdong, China.
3. College of Food Science and Engineering, South China University of Technology, Guangzhou 510640, Guangdong, China.
Publication Type:Journal Article
Keywords: Anti-inflammatory activity; Antioxidant activity; Fermentation; Ferulic acid; Fungal identification; Oxidative hemolysis
MeSH: Animals; Anti-Inflammatory Agents; metabolism; pharmacology; Antioxidants; metabolism; pharmacology; Aspergillus niger; genetics; isolation & purification; metabolism; Coumaric Acids; metabolism; pharmacology; DNA, Fungal; analysis; Dietary Fiber; microbiology; Erythrocytes; drug effects; metabolism; Fermentation; Hep G2 Cells; Humans; Interleukin-6; metabolism; Lipopolysaccharides; pharmacology; Mice; RAW 264.7 Cells; Sheep; Tumor Necrosis Factor-alpha; metabolism
From: Biomedical and Environmental Sciences 2019;32(1):11-21
CountryChina
Language:English
Abstract: OBJECTIVE:A strain of Aspergillus niger (A. niger), capable of releasing bound phenolic acids from wheat bran, was isolated. This strain was identified by gene sequence identification. The antioxidant and anti-inflammatory capacity of ferulic acid released from wheat bran by this A. niger strain (FA-WB) were evaluated.
METHODS:Molecular identification techniques based on PCR analysis of specific genomic sequences were conducted; antioxidant ability was examined using oxygen radical absorbance capacity (ORAC), cellular antioxidant activity (CAA) assays, and erythrocyte hemolysis assays. RAW264.7 cells were used as a model to detect anti-inflammatory activity.
RESULTS:The filamentous fungal isolate was identified to be A. niger. ORAC and CAA assay showed that FA-WB had better antioxidant activity than that of the ferulic acid standard. The erythrocyte hemolysis assay results suggested that FA-WB could attenuate AAPH-induced oxidative stress through inhibition of reactive oxy gen species (ROS) generation. FA-WB could significantly restore the AAPH-induced increase in intracellular antioxidant enzyme activities to normal levels as well as inhibit the intracellular malondialdehyde formation. TNF-a, IL-6, and NO levels indicated that FA-WB can inhibit the inflammation induced by lipopolysaccharide (LPS).
CONCLUSION:Ferulic acid released from wheat bran by a new strain of A. niger had good anti-inflammatory activity and better antioxidant ability than standard ferulic acid.