Antioxidant Potential of Four Species of Natural Product and Therapeutic Strategies for Cancer through Suppression of Viability in the Human Multiple Myeloma Cell Line U266.

Fatma GUESMI; Issam SAIDI; Rawya SOUSSI; Najla HFAIEDH; Ahmed LANDOULSI

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Antioxidant Potential of Four Species of Natural Product and Therapeutic Strategies for Cancer through Suppression of Viability in the Human Multiple Myeloma Cell Line U266.

Author: Fatma GUESMI ¹ ; Issam SAIDI ² ; Rawya SOUSSI ³ ; Najla HFAIEDH ⁴ ; Ahmed LANDOULSI ¹
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1. Laboratory of Biochemistry and Molecular Biology, Faculty of Sciences of Bizerte, University of Carthage, 1054, Tunisia.
2. Research Unit of Macromolecular Biochemistry and Genetics, Faculty of Sciences of Gfasa, 2112, Tunisia.
3. Laboratory of Animal Eco Physiology, Faculty of Sciences of Gafsa, 2112, Tunisia.
4. Laboratory of Integrated Physiology, Faculty of Science of Bizerte, University of Carthage, 1054, Tunisia.
Publication Type:Journal Article
Keywords: Antibacterial effect; DPPH; FRAP; Methanolic fractions; Phenolic content; U266
MeSH: Anti-Bacterial Agents; pharmacology; Antineoplastic Agents, Phytogenic; pharmacology; Antioxidants; pharmacology; Bacteria; drug effects; growth & development; Biological Products; pharmacology; Cell Line, Tumor; Cell Survival; drug effects; Humans; Magnoliopsida; chemistry; Multiple Myeloma; Phytochemicals; analysis; pharmacology; Plant Extracts; chemistry; pharmacology
From: Biomedical and Environmental Sciences 2019;32(1):22-33
CountryChina
Language:English
Abstract: OBJECTIVE:This research aimed to evaluate the protective effects of bioactive compounds such as phenolic acids, flavonoids, and tannins present in four species extracted with methanol.
METHODS:The total phenolic content of the methanolic extracts was measured spectrophotometrically. The effect of the extracts on cell viability in U266 cells was measured. The effects of extracts on free radical scavenging were assessed by the DPPH test and FRAP assay. Antibacterial effects of the natural products in this report were investigated by using the disc diffusion method.
RESULTS:Our results clearly demonstrated that the methanolic extracts were characterized by a high amount of phenolic compounds. It has been speculated that ME-TA and ME-TAl exhibit a significant (P < 0.05) and dose-dependent antiradical potential. The exposure of cells to high doses of extracts almost completely suppressed cell growth in vitro. ME-TA and ME-TAl showed significant cytotoxic effects at a concentration of 100 μg/mL in the U266 cell line. ME-TAl and ME-CF inhibited the growth of B. subtilis and S. aureus, respectively, to the same extent as 10 μg/μL of chloramphenicol at a concentration of 1 mg/mL.
CONCLUSION:Overall, these results suggest that plants used in traditional medicine have a novel application as free radical scavengers, bacterial inhibitors and tumor suppressors.