Behavioral Abnormality along with NMDAR-related CREB Suppression in Rat Hippocampus after Shortwave Exposure.
- Author:
Chao YU
1
;
Yan Xin BAI
1
;
Xin Ping XU
1
;
Ya Bing GAO
1
;
Yan Hui HAO
1
;
Hui WANG
1
;
Sheng Zhi TAN
1
;
Wen Chao LI
1
;
Jing ZHANG
1
;
Bin Wei YAO
1
;
Ji DONG
1
;
Li ZHAO
1
;
Rui Yun PENG
1
Author Information
- Publication Type:Journal Article
- Keywords: CREB; Hippocampus; Learning and memory; NMDAR; Shortwave exposure
- MeSH: Animals; Cyclic AMP Response Element-Binding Protein; genetics; metabolism; Dose-Response Relationship, Radiation; Electroencephalography; radiation effects; Hippocampus; radiation effects; Male; Memory; radiation effects; Nissl Bodies; physiology; radiation effects; Radio Waves; adverse effects; Random Allocation; Rats; Rats, Wistar; Receptors, N-Methyl-D-Aspartate; genetics; metabolism; Spatial Learning; radiation effects
- From: Biomedical and Environmental Sciences 2019;32(3):189-198
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To estimate the detrimental effects of shortwave exposure on rat hippocampal structure and function and explore the underlying mechanisms.
METHODS:One hundred Wistar rats were randomly divided into four groups (25 rats per group) and exposed to 27 MHz continuous shortwave at a power density of 5, 10, or 30 mW/cm2 for 6 min once only or underwent sham exposure for the control. The spatial learning and memory, electroencephalogram (EEG), hippocampal structure and Nissl bodies were analysed. Furthermore, the expressions of N-methyl-D-aspartate receptor (NMDAR) subunits (NR1, NR2A, and NR2B), cAMP responsive element-binding protein (CREB) and phosphorylated CREB (p-CREB) in hippocampal tissue were analysed on 1, 7, and 14 days after exposure.
RESULTS:The rats in the 10 and 30 mW/cm2 groups had poor learning and memory, disrupted EEG oscillations, and injured hippocampal structures, including hippocampal neurons degeneration, mitochondria cavitation and blood capillaries swelling. The Nissl body content was also reduced in the exposure groups. Moreover, the hippocampal tissue in the 30 mW/cm2 group had increased expressions of NR2A and NR2B and decreased levels of CREB and p-CREB.
CONCLUSION:Shortwave exposure (27 MHz, with an average power density of 10 and 30 mW/cm2) impaired rats' spatial learning and memory and caused a series of dose-dependent pathophysiological changes. Moreover, NMDAR-related CREB pathway suppression might be involved in shortwave-induced structural and functional impairments in the rat hippocampus.
