NANOG Alleviates the Damage of Human Hair Follicle Mesenchymal Stem Cells Caused by H2O2 through Activation of AKT Pathway.
- Author:
Jia Hong SHI
1
;
Kui Yang ZUO
1
;
Ying Yao ZHANG
1
;
Bo WANG
1
;
Xing HAN
1
;
Ao Bo LIAN
1
;
Jin Yu LIU
1
Author Information
- Publication Type:Journal Article
- Keywords: AKT; Hair follicles; Hydrogen peroxide; Mesenchymal stem cells; NANOG homeobox protein
- MeSH: Cell Survival; Drug Evaluation, Preclinical; Hair Follicle; cytology; Humans; Hydrogen Peroxide; Lentivirus; Mesenchymal Stem Cells; drug effects; metabolism; Nanog Homeobox Protein; metabolism; pharmacology; Oxidative Stress; drug effects; Phosphatidylinositol 3-Kinases; metabolism; Proto-Oncogene Proteins c-akt; metabolism; Signal Transduction
- From: Biomedical and Environmental Sciences 2019;32(4):272-280
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To explore the protective effect of NANOG against hydrogen peroxide (H2O2) -induced cell damage in the human hair follicle mesenchymal stem cells (hHF-MSCs).
METHODS:NANOG was expressed from a lentiviral vector, pLVX-IRES-ZsGreen. NANOG hHF-MSCs and vector hHF-MSCs were treated with 400 μmol/L hydrogen peroxide (H2O2) for 2 h, the cell survival rate, cell morphology, ROS production, apoptosis and expression of AKT, ERK, and p21 were determined and compared.
RESULTS:Our results showed that NANOG could activate AKT and upregulate the expression of p-AKT, but not p-ERK. When treated with 400 μmol/L H2O2, NANOG hHF-MSCs showed higher cell survival rate, lower ROS production and apoptosis, higher expression of p-AKT, higher ratio of p-AKT/AKT.
CONCLUSION:Our results suggest that NANOG could protect hHF-MSCs against cell damage caused by H2O2 through activating AKT signaling pathway.
