2,3,7,8-Tetrachlorodibenzo-p-dioxin Promotes Proliferation of Astrocyte Cells via the Akt/STAT3/Cyclin D1 Pathway.

Chang Yue WU; Kai Zhi Yin; Yan Zhang; Man Jiao; Xin Yuan Zhao; Qi Yun WU

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2,3,7,8-Tetrachlorodibenzo-p-dioxin Promotes Proliferation of Astrocyte Cells via the Akt/STAT3/Cyclin D1 Pathway.

Author: Chang Yue WU ¹ ; Kai Zhi YIN ^{2
,

3} ; Yan ZHANG ⁴ ; Man JIAO ⁴ ; Xin Yuan ZHAO ⁵ ; Qi Yun WU ⁴
Author Information

1. Medical Colledge, Nantong University, Nantong 226001, Jiangsu, China.
2. Department of Nutrition and Food Hygiene, School of Public Health, Nantong University, Nantong 226019, Jiangsu, China
3. Xishan District Center for Disease Control and Preventive, Wuxi 204011, Jiangsu, China.
4. Department of Nutrition and Food Hygiene, School of Public Health, Nantong University, Nantong 226019, Jiangsu, China.
5. Department of Occupational Medicine and Environmental Toxicology, School of Public Health, Nantong University, Nantong 226019, Jiangsu, China.
Publication Type:Journal Article
Keywords: 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD); Akt; Astrocytes; Cyclin D1; Proliferation; STAT3
MeSH: Animals; Animals, Newborn; Astrocytes; drug effects; Cell Proliferation; drug effects; Cyclin D1; metabolism; Environmental Pollutants; toxicity; Neurotoxins; toxicity; Polychlorinated Dibenzodioxins; toxicity; Proto-Oncogene Proteins c-akt; metabolism; Rats, Sprague-Dawley; STAT3 Transcription Factor; metabolism
From: Biomedical and Environmental Sciences 2019;32(4):281-290
CountryChina
Language:English
Abstract: OBJECTIVE:The compound 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), a persistent organic pollutant, is harmful to the nervous system, but its effects on the brain are still unclear. This study aimed to investigate the effects of TCDD on astrocytes proliferation and underlying molecular mechanism.
METHODS:The cell proliferation was measured by EdU-based proliferation assay and PI staining by flow cytometry. Protein expression levels were detected by Western blotting. Immunofluorescence, cytoplasmic and nuclear fractions separation were used to assess the distribution of signal transducer and activator of transcription 3 (STAT3).
RESULTS:C6 cells treated with 10 and 50 nmol/L TCDD for 24 h showed significant promotion of the proliferation of. The exposure to TCDD resulted in the upregulation in the expression levels of phosphorylated protein kinase B (p-Akt), phosphorylated STAT3, and cyclin D1 in a dose- and time-dependent manner. The inhibition of Akt expression with LY294002 or STAT3 expression with AG490 abolished the TCDD-induced cyclin D1 upregulation and cell proliferation. Furthermore, LY294002 suppressed the activation of STAT3. Finally, TCDD promoted the translocation of STAT3 from the cytoplasm to the nucleus, and LY294002 treatment blocked this effect.
CONCLUSION:TCDD exposure promotes the proliferation of astrocyte cells via the Akt/STAT3/cyclin D1 pathway, leading to astrogliosis.