Di (2-ethylhexyl) phthalate Disorders Lipid Metabolism via TYK2/STAT1 and Autophagy in Rats.
- Author:
Yue Zhu ZHANG
1
;
Zhao Ming ZHANG
1
;
Li Ting ZHOU
1
;
Jian ZHU
1
;
Xiao Han ZHANG
1
;
Wen QI
1
;
Shuang DING
1
;
Qi XU
1
;
Xu HAN
1
;
Ya Ming ZHAO
1
;
Xin Yue SONG
1
;
Tian Yang ZHAO
1
;
Lin YE
1
Author Information
- Publication Type:Journal Article
- Keywords: Autophagy; DEHP; High-fat diet; Lipid metabolism; STAT1; TYK2
- MeSH: Adipose Tissue; drug effects; metabolism; Animals; Autophagy; drug effects; Body Weight; drug effects; Diet, High-Fat; adverse effects; Diethylhexyl Phthalate; toxicity; Endocrine Disruptors; toxicity; Female; Lipid Metabolism; drug effects; Lipid Metabolism Disorders; chemically induced; Liver; drug effects; metabolism; Male; Rats, Wistar; STAT1 Transcription Factor; metabolism; TYK2 Kinase; metabolism
- From: Biomedical and Environmental Sciences 2019;32(6):406-418
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:Previous studies have indicated that the plasticizer di (2-ethylhexyl) phthalate (DEHP) affects lipid accumulation; however, its underlying mechanism remains unclear. We aim to clarify the effect of DEHP on lipid metabolism and the role of TYK2/STAT1 and autophagy.
METHODS:In total, 160 Wistar rats were exposed to DEHP [0, 5, 50, 500 mg/(kg•d)] for 8 weeks. Lipid levels, as well as mRNA and protein levels of TYK2, STAT1, PPARγ, AOX, FAS, LPL, and LC3 were detected.
RESULTS:The results indicate that DEHP exposure may lead to increased weight gain and altered serum lipids. We observed that DEHP exposure affected liver parenchyma and increased the volume or number of fat cells. In adipose tissue, decreased TYK2 and STAT1 promoted the expression of PPARγ and FAS. The mRNA and protein expression of LC3 in 50 and 500 mg/(kg•d) groups was increased significantly. In the liver, TYK2 and STAT1 increased compensatorily; however, the expression of FAS and AOX increased, while LPL expression decreased. Joint exposure to both a high-fat diet and DEHP led to complete disorder of lipid metabolism.
CONCLUSION:It is suggested that DEHP induces lipid metabolism disorder by regulating TYK2/STAT1. Autophagy may play a potential role in this process as well. High-fat diet, in combination with DEHP exposure, may jointly have an effect on lipid metabolism disorder.
