Intranasal Immunization Using CTA1-DD as a Mucosal Adjuvant for an Inactivated Influenza Vaccine.

Xue Ting Fan; Yun Long Wang; Qiu Dong SU; Feng Qiu; Yao YI; Zhi Yuan Jia; Da Yan Wang; Kun Qin; Ye Ning Zou; Sheng Li BI; Li Ping Shen

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Intranasal Immunization Using CTA1-DD as a Mucosal Adjuvant for an Inactivated Influenza Vaccine.

Author: Xue Ting FAN ¹ ; Yun Long WANG ² ; Qiu Dong SU ¹ ; Feng QIU ¹ ; Yao YI ¹ ; Zhi Yuan JIA ¹ ; Da Yan WANG ¹ ; Kun QIN ¹ ; Ye Ning ZOU ³ ; Sheng Li BI ¹ ; Li Ping SHEN ¹
Author Information

1. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
2. Henan Bioengineering Research Center, Zhengzhou 4500466, Henan, China.
3. Sinovac Biotech Co., Ltd., Beijing 100085, China.
Publication Type:Journal Article
Keywords: Adjuvants; H3N2; Influenza; Mucosal immunization; Split vaccine
MeSH: Adjuvants, Immunologic; Administration, Intranasal; Animals; Cholera Toxin; Female; Immunity, Humoral; Influenza A Virus, H3N2 Subtype; immunology; Influenza Vaccines; Mice, Inbred BALB C; Nasal Mucosa; immunology; Random Allocation; Recombinant Fusion Proteins
From: Biomedical and Environmental Sciences 2019;32(7):531-540
CountryChina
Language:English
Abstract: OBJECTIVE:To evaluate the effect of intranasal immunization with CTA1-DD as mucosal adjuvant combined with H3N2 split vaccine.
METHODS:Mice were immunized intranasally with PBS (negative control), or H3N2 split vaccine (3 μg/mouse) alone, or CTA1-DD (5 μg/mouse) alone, or H3N2 split vaccine (3 μg/mouse) plus CTA1-DD (5 μg/mouse). Positive control mice were immunized intramuscularly with H3N2 split vaccine (3 μg/mouse) and alum adjuvant. All the mice were immunized twice, two weeks apart. Then sera and mucosal lavages were collected. The specific HI titers, IgM, IgG, IgA, and IgG subtypes were examined by ELISA. IFN-γ and IL-4 were test by ELISpot. In addition, two weeks after the last immunization, surivival after H3N2 virus lethal challenge was measured.
RESULTS:H3N2 split vaccine formulated with CTA1-DD could elicit higher IgM, IgG and hemagglutination inhibition titers in sera. Furthermore, using CTA1-DD as adjuvant significantly improved mucosal secretory IgA titers in bronchoalveolar lavages and vaginal lavages. Meanwhile this mucosal adjuvant could enhance Th-1-type responses and induce protective hemagglutination inhibition titers. Notably, the addition of CTA1-DD to split vaccine provided 100% protection against lethal infection by the H3N2 virus.
CONCLUSION:CTA1-DD could promote mucosal, humoral and cell-mediated immune responses, which supports the further development of CTA1-DD as a mucosal adjuvant for mucosal vaccines.