Comparative Toxicity of Nanomaterials to Air-blood Barrier Permeability Using an In Vitro Model.

Kang Feng Zhao; Yu Qing Song; Rui Hua Zhang; Xiao Yan Yang; Bo Sun; Zhi Quan Hou; Xiao Ping PU; Hong Xing Dai; Xue Tao Bai

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Comparative Toxicity of Nanomaterials to Air-blood Barrier Permeability Using an In Vitro Model.

Author: Kang Feng ZHAO ¹ ; Yu Qing SONG ² ; Rui Hua ZHANG ³ ; Xiao Yan YANG ¹ ; Bo SUN ¹ ; Zhi Quan HOU ⁴ ; Xiao Ping PU ⁵ ; Hong Xing DAI ⁴ ; Xue Tao BAI ¹
Author Information

1. Department of Environmental Toxicology, National Institute of Environmental Health, Chinese Center for Disease Control and Prevention, Beijing 100021, China.
2. Peking Union Medical College, Beijing 100730, China.
3. Research Institute of Chemical Defence, Beijing 100083, China.
4. Department of Chemical Engineering, School of Environment and Energy Engineering, Beijing University of Technology, Beijing 100124, China.
5. Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China.
Publication Type:Journal Article
Keywords: Air-blood barrier; In vitro model; Metal-containing nanoparticles (MNPs); Permeability; Toxicity screening
MeSH: A549 Cells; Blood-Air Barrier; metabolism; Epithelium; metabolism; Humans; Metal Nanoparticles; toxicity; Particle Size; Permeability
From: Biomedical and Environmental Sciences 2019;32(8):602-613
CountryChina
Language:English
Abstract: OBJECTIVE:To comparatively study the toxicity of four metal-containing nanoparticles (MNPs) and their chemical counterparts to the air-blood barrier (ABB) permeability using an in vitro model.
METHODS:ABB model, which was developed via the co-culturing of A549 and pulmonary capillary endothelium, was exposed to spherical CuO-NPs (divided into CuO-40, CuO-80, and CuO-100 based on particle size), nano-Al2O3 (sheet and short-rod-shaped), nano-ZnO, nano-PbS, CuSO4, Al2(SO4)3, Zn(CH3COO)2, and Pb(NO3)2 for 60 min. Every 10 min following exposure, the cumulative cleared volume (ΔTCL) of Lucifer yellow by the model was calculated. A clearance curve was established using linear regression analysis of ΔTCL versus time. Permeability coefficient (P) was calculated based on the slope of the curve to represent the degree of change in the ABB permeability.
RESULTS:The results found the increased P values of CuO-40, CuO-80, sheet, and short-rod-shaped nano-Al2O3, Al2(SO4)3, and Pb(NO3)2. Among them, small CuO-40 and CuO-80 were stronger than CuO-100 and CuSO4; no difference was observed between Al2(SO4)3 and sheet and short-rod-shaped nano-Al2O3; and nano-PbS was slightly weaker than Pb(NO3)2. So clearly the MNPs possess diverse toxicity.
CONCLUSION:ABB permeability abnormality means pulmonary toxicity potential. More studies are warranted to understand MNPs toxicity and ultimately control the health hazards.