Neurotrophin 3 promotes osteogenic differentiation of human dental follicle cells.

Lei Wei; Ma Yue; Ren Aishu

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Neurotrophin 3 promotes osteogenic differentiation of human dental follicle cells.

Author: Lei WEI ^{1
,

2} ; Ma YUE ^{1
,

2} ; Ren AISHU ^{1
,

3}
Author Information

1. Dept. of Orthodontics, Stomatological Hospital of Chongqing Medical University, Chongqing 401147, China
2. Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing 401147, China.
3. Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China.
Publication Type:Journal Article
Keywords: human dental follicle cells; neurotrophin 3; osteogenic differentiation
MeSH: Alkaline Phosphatase; Bone Morphogenetic Protein 2; metabolism; Cell Differentiation; Cells, Cultured; Dental Sac; Humans; Mesenchymal Stem Cells; Neurotrophin 3; pharmacology; Osteocalcin; metabolism; Osteogenesis
From: West China Journal of Stomatology 2018;36(1):23-28
CountryChina
Language:Chinese
Abstract: OBJECTIVE:This study aims to investigate the effect of neurotrophin 3 (NT-3) on the osteogenic differentiation of human dental follicle cells (hDFCs).
METHODS:hDFCs were isolated and cultured in vitro. Immunocytochemical staining was used to identify the origin of hDFCs. The effects of different NT-3 concentrations on hDFCs proliferation were detected by using CCK-8 assay. The alkaline phosphatase (ALP) activities and mRNA expression levels of bone morphogenetic protein-2 (BMP-2) and osteocalcin (OCN) were determined to investigate the effects of NT-3 on hDFCs osteogenesis. The difference in the number of mineralized nodules was detected using alizarin red staining.
RESULTS:Vimentin and cytokeratin staining results showed that hDFCs originated from the mesenchymal cells. NT-3 exerted no evident effect on hDFCs proliferation. The ALP activity and the BMP-2 and OCN mRNA expression levels of hDFCs were signiﬁcantly improved under treatment with different NT-3 concentrations (25, 50, and 100 ng·mL ⁻¹) compared with those in the control group. BMP-2 and OCN mRNA relative expression levels of hDFCs reached the highest when the NT-3 concentration was 100 ng·mL ⁻¹. The number of mineralized nodules reached the maximum when the hDFCs were treated with 50 and 100 ng·mL ⁻¹ NT-3.
CONCLUSIONS:Appropriate mass concentration of NT-3 can promote the osteogenic differentiation of hDFCs.