K (lysine) acetyltransferase 2A affects the osteogenic differentiation of periodontal ligament stem cells through the canonical Wnt pathway.

Guo WUCHENG; Cheng JIELI; Yang ZHENGYI; Zhang YI; He ENLIANG; Qian JUN; Song JINGJING; Sun JIN; Yuan LIN

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K (lysine) acetyltransferase 2A affects the osteogenic differentiation of periodontal ligament stem cells through the canonical Wnt pathway.

VernacularTitle:赖氨酸乙酰基转移酶2A通过经典Wnt通路影响牙周膜干细胞成骨分化
Author: Guo WUCHENG ¹ ; Cheng JIELI ¹ ; Yang ZHENGYI ¹ ; Zhang YI ¹ ; He ENLIANG ¹ ; Qian JUN ¹ ; Song JINGJING ¹ ; Sun JIN ² ; Yuan LIN ¹
Author Information

1. Dept. of Stomatology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China.
2. Stomatological Disease Center, Shenzhen Maternal and Child Health Care Hospital, Southern Medical University, Shenzhen 518048, China.
Publication Type:Journal Article
Keywords: acetyltransferase; classical Wnt pathway; osteogenic differentiation; periodontal ligament stem cells; periodontitis
MeSH: Acetyltransferases; Cell Differentiation; Cells, Cultured; Histone Acetyltransferases; metabolism; Humans; Lysine; Osteogenesis; Periodontal Ligament; metabolism; Periodontitis; metabolism; Stem Cells; Wnt Signaling Pathway
From: West China Journal of Stomatology 2018;36(1):39-45
CountryChina
Language:Chinese
Abstract: OBJECTIVE:This study aims to investigate the mechanism of K (lysine) acetyltransferase 2A (KAT2A) regulation and control on the osteogenic differentiation of periodontal ligament stem cells (PDLSCs).
METHODS:The expression levels of KAT2A in PDLSCs were compared from each generation of the normal (H-PDLSCs) and periodontitis tissues (P-PDLSCs). The influences of KAT2A gene interference on the osteogenic differentiation of PDLSCs were also detected. In addition, the influences of the KAT2A gene interference to the canonical Wnt pathway and ligands were detected. The upstream and down-stream relationships between KAT2A and canonical Wnt pathway were also determined.
RESULTS:The decreased expression of KAT2A in PDLSCs from the inflammatory tissue in each generation was compared with that in PDLSCs from the healthy tissue, and the difference was statistically significant (P<0.05). When the KAT2A gene was disrupted, the osteogenesis ability of PDLSC was declined, and the difference was statistically significant (P<0.05). The canonical Wnt pathway was activated, and the antagonist Dickkopf-1 (DKK-1) was reduced. After the DKK-1 addition, the osteogenic differentiation of the disturbed PDLSCs was recovered, and KAT2A was unaffected.
CONCLUSIONS:The KAT2A expression in PDLSCs was decreased because of perio-dontitis. The classical Wnt pathway was activated to inhibit the osteogenic differentiation of the cells.