Experimental research on Arginine-gingipain A gene vaccine from Porphyromonas gingivalis that prevents peri-implantitis in Beagle dogs.
- Author:
Li CHUANHUA
1
;
Wang ZHIFENG
2
;
Zhu LINA
2
;
Fan XIN
3
;
Lan JING
1
Author Information
- Publication Type:Journal Article
- Keywords: Arginine-gingipains; Porphyromonas gingivalis; dental implant; gene vaccine; peri-implantitis
- MeSH: Adhesins, Bacterial; therapeutic use; Alveolar Bone Loss; Animals; Arginine; Cysteine Endopeptidases; therapeutic use; Dental Implants; Dogs; Peri-Implantitis; prevention & control; Porphyromonas gingivalis; chemistry; Vaccines; therapeutic use
- From: West China Journal of Stomatology 2018;36(1):76-81
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:This study aims to use Arginine-gingipain A gene vaccine (pVAX1-rgpA) to immunize adult Beagle dogs and to evaluate its effect during peri-implantitis progression and development.
METHODS:Plasmid pVAX1-rgpA was constructed. The second and third bilateral mandible premolars of 15 adult Beagle dogs were extracted, and the implants were placed immediately. After 3 months, the animals were randomly divided into groups A, B, and C. Afterward, the animals were immunized thrice with plasmid pVAX1-rgpA, with heat-killed Porphyromonas gingivalis, or pVAX1, respectively. IgG in the serum and secretory IgA (sIgA) in saliva were quantitatively analyzed by enzyme-linked immunosorbent assay before and after 2 weeks of immunization. Peri-implantitis was induced with cotton ligatures fixed around the neck of implants. Probing depth (PD) and bleeding on probing were recorded. All animals were sacrificed after ligaturation for 6 weeks. Decalcified sections with thickness of 50 μm were prepared and dyed with methylene blue to observe the bone phenotype around implants.
RESULTS:Levels of serum IgG and sIgA in saliva were higher in groups A and B after immunization than before the process (P<0.05) and higher than those in group C (P<0.05). However, no difference was observed between groups A and B (P>0.05). At 4 and 6 weeks after ligaturation, PD of the ligatured side in group C was higher than that in groups A and B (P<0.05). On the other hand, no difference was identified between groups A and B (P>0.05). Bone loss in group A was significantly lower than that of the other groups (P<0.05). Abundant inflammatory cells and bacteria were present in the bone loss area around the implants in the three groups, as identified through hard tissue section observation. However, group C presented the most number of inflammatory cells and bacteria in the bone loss area around the implants.
CONCLUSIONS:IgG and sIgA can be generated by immunity with rgpA DNA vaccine, which can significantly slow down bone loss during experimental peri-implantitis in dogs.