Disheveled 2 around the implant at an early stage of hyperlipidemic rats.
- Author:
Dong XIAOFEI
1
;
Wang HUI
2
;
Lan JING
1
Author Information
- Publication Type:Journal Article
- Keywords: Wnt signal pathway; bone remodeling; disheveled 2; hyperlipidemia
- MeSH: Animals; Bone Remodeling; Cathepsin K; metabolism; Core Binding Factor Alpha 1 Subunit; metabolism; Dishevelled Proteins; metabolism; Osteoblasts; Rats; Rats, Wistar
- From: West China Journal of Stomatology 2018;36(1):82-86
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:This study aims to investigate the expression of disheveled 2 (Dvl2) around the implant of hyperlipidemic rats at an early stage after the implantation.
METHODS:A total of 24 Wistar rats were divided equally into the experimental group fed with high-fat diet group and control group fed with a normal diet. After 8 weeks, the serum lipid levels were detected, and rats received implants in the femur metaphysis. Rats were sacrificed at 1, 3, and 5 days after implantation, and the bones around implants were obtained. Methylene blue-acid fuchsin staining was performed to observe the implant-bone interface. Real-time polymerase chain reaction was performed on runt-related transcription factor 2 (Runx2), cathepsin K (CatK), and Dvl2. Dvl2 Western blot or immunoprecipitation, phosphorylation, and ubiquitination were also conducted.
RESULTS:In the experimental group, less osteoblasts, lower expression of Runx2 and Dvl2, and lower Dvl2 phosphorylation (P<0.05) than those of the control group were observed; furthermore, the CatK expression and Dvl2 ubiquitination were higher than those in the control group (P<0.05).
CONCLUSIONS:Hyperlipidemia may suppress bone remodeling around the implant at an early stage by Dvl2 down-regulation, phosphorylation, and up-regulated ubiquitination.
