Comparison of contents of three active ingredients in Bletilla striata from different sources.
10.19540/j.cnki.cjcmm.20190505.105
- Author:
Fu-Sheng JIANG
1
;
Xu-Ting SHEN
2
;
Yue YAO
2
;
Bin DING
1
;
Mei-Ya LI
3
;
Zhi-Shan DING
2
;
Gui-Yuan LYU
4
Author Information
1. College of Life Science,Zhejiang Chinese Medical University Hangzhou 310053,China.
2. College of Medical Technology,Zhejiang Chinese Medical University Hangzhou 310053,China.
3. Academy of Chinese medicine,Zhejiang Chinese Medical University Hangzhou 310053,China.
4. College of Pharmacy,Zhejiang Chinese Medical University Hangzhou 310053,China.
- Publication Type:Journal Article
- Keywords:
Bletilla striata;
UPLC;
coelonin;
content determination
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
chemistry;
Orchidaceae;
chemistry;
Quality Control
- From:
China Journal of Chinese Materia Medica
2019;44(13):2762-2767
- CountryChina
- Language:Chinese
-
Abstract:
In order to understand the difference of contents of coelonin,batatasin Ⅲ and 3'-O-methylbatatasin Ⅲ in 60 different sources of Bletilla striata planted under the same conditions. UPLC method was used and the analysis was performed on a Waters ACQUITY UPLC BEH C18 column( 2. 1 mm×100 mm,1. 7 μm),eluted with acetonitril-0. 1% formic acid solution by gradient. The flow rate was 0. 208 m L·min-1,the detection wavelength was 270 nm,the column temperature was 35 ℃ and the injection volume was 4μL. Under the above chromatographic conditions,the three components can be separated well with good linearity in the range of 0. 156-5. 000 mg·L-1. The average contents of coelonin,batatasin Ⅲ and 3'-O-methylbatatasin Ⅲ were( 0. 116 ± 0. 071) %,( 0. 386 ±0. 185) % and( 0. 086±0. 034) %,respectively. After planting for two years under the same conditions,there was no significant difference in chemical composition among different sources and varieties,but the contents of the three components had some regional differences,which indicated that the western region was higher than the eastern region,while the contents of coelonin and batatasin Ⅲ in B.sinensis were slightly higher than those in B. striata. The chromatographic method above is simple,stable and reproducible,and can be used for quantitative analysis of three components. The content analysis of different sources of B. striata can provide reference for future B. striata breeding and quality control.
