Preliminary study on response and its mechanism of ginsenoside biosynthesis in Panax ginseng to water regulation.
10.19540/j.cnki.cjcmm.20190323.103
- Author:
Yan GAO
1
;
Tao ZHANG
1
;
Xue-Ping KANG
2
;
Mei HAN
1
;
Li-Min YANG
1
Author Information
1. College of Chinese materials,Jilin Agricultural University Changchun 130118,China.
2. Yanbian Korean Autonomous Prefecture Academy of Agricultural Sciences Yanbian 133001,China.
- Publication Type:Journal Article
- Keywords:
antioxidant enzyme system;
fluorescence quantitative PCR;
gene expression;
ginseng saponin;
water regulation
- MeSH:
Chromatography, High Pressure Liquid;
Ginsenosides;
biosynthesis;
Panax;
physiology;
Water;
physiology
- From:
China Journal of Chinese Materia Medica
2019;44(13):2768-2776
- CountryChina
- Language:Chinese
-
Abstract:
The study is aimed to explore the effect of soil moisture content on ginsenoside biosynthesis and explain its mechanism from the perspectives of antioxidant enzyme system and gene expression of key enzymes in the pathway of ginsenoside synthesis. In the study,two years old Panax ginseng was used as the experimental material and three moisture gradient,40% of saturated water content( W1),60%( W2),80%( W3) were set up. The content of 11 monomeric saponins were determined by HPLC. With GAPDH as a reference gene,six key enzymes( HMGR,SS,β-AS,CYP716 A47,CYP716 A52 v2,CYP716 A53 v2) in ginseng saponin synthesis pathway expression were analyzed by fluorescent quantitative PCR and the activities of superoxide dismutase( SOD),peroxidase( POD),catalase( CAT) activity and MDA content were also determined. With the increase of soil water,the content of ginseng saponin and biomass showed an increasing trend. PPD( Rb1,Rc,Rb2,Rd,Rh2,Rb3,Rg3),PPT( Rg1,Re,Rf) ginsenoside,Ro and total ginsenoside reached the maximum value on August 30,were 9.92,5.48,0.63 mg·g-1,respectively. During the whole regulation period,the antioxidant activity of W3 was greater than that of W1,and the MDA content was less than that of W1. At W3,expression levels of β-AS,CYP716 A47 and CYP716 A53 v2 showed an increasing trend,while HMGR and SS genes showed relatively stable expression levels under various water conditions. According to the correlation analysis,HMGR and SS genes in the W3 treatment group were significantly positively correlated with PPD,PPT ginsenoside and Ro,CYP716 A52 v2 gene was significantly positively correlated with Ro,and CYP716 A47 gene was significantly positively correlated with PPD ginsenoside. There was a significant positive correlation between β-AS gene and PPD ginsenoside in W1 and W2 treatment. Therefore,W3 is the optimum moisture content,ginseng total saponins and monomer saponin content is the highest,the gene closely correlation with content of saponins and more conducive to the accumulation of ginsenosides.
