Establishment of extraction method for Gardeniae Fructus extract and its application in evaluating different Gardeniae Fructus decoction pieces.
10.19540/j.cnki.cjcmm.20190321.309
- Author:
Xue ZHANG
1
;
Yun WANG
1
;
Qing-Hao WANG
2
;
Guo-You WANG
2
;
De-Peng LIU
3
;
Yin-Lian MA
1
;
Yan SONG
4
;
Yu-Lian ZHANG
4
;
Ting-Ting LYU
4
;
Cun ZHANG
5
Author Information
1. Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
2. Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China College of Pharmacy,Henan University of Chinese Medicine Zhengzhou 450008,China.
3. Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China College of Pharmacy,Anhui University of Chinese Medicine Hefei 230000,China.
4. SPH Huayu Chinese Herbs Co.,Ltd. Shanghai 200002,China.
5. Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China College of Pharmacy,Henan University of Chinese Medicine Zhengzhou 450008,China College of Pharmacy,Anhui University of Chinese Medicine Hefei 230000,China.
- Publication Type:Journal Article
- Keywords:
Gardeniae Fructus;
crocin-Ⅰ;
crocin-Ⅱ;
extract;
gardoside;
geniposide
- MeSH:
Drugs, Chinese Herbal;
analysis;
Fruit;
chemistry;
Gardenia;
chemistry;
Plant Extracts;
analysis
- From:
China Journal of Chinese Materia Medica
2019;44(13):2792-2798
- CountryChina
- Language:Chinese
-
Abstract:
In this experiment,the gradation analysis method was used to evaluate the quality of different pieces of Gardeniae Fructus through the extraction rate difference and the difference analysis of the main components in the extract. In this experiment cold-dip and hot-dip methods were used to compare the yield of Gardeniae Fructus extract and the content of chemical constituents with water,25%,50%,75% and 95% ethanol fractions. By weighted calculation,the optimal extraction method of Gardeniae Fructus was determined,and this was verified by practical application. RESULTS:: showed that for the water-soluble extract,cold dip method was better than the hot dip method; and for alcohol-soluble extract,75% ethanol under cold dip method was best. The verification results showed that water-soluble extracts under cold dip methods could be used to significantly distinguish the raw Gardeniae Fructus( GF) and processed( stir-baked) GF( GFP) collected from the market. Meanwhile,this method could be also used to distinguish the same batch of GF,GFP and carbonized GF( GFC) with significant differences,respectively( P<0. 05). Ethanol-soluble extract can be used to clearly distinguish GFP and GFC pieces in the same batch( P<0. 05). The results of content determination showed that the variation coefficient of components in GF processed products was higher than that in extracts,and the content of hydroxygeniposide was the most significant component between GF and its processed products. It is suggested that the method of water-soluble extract of GF and the determination of the content of gardoside should be combined together to evaluate the quality of GF and its heat processed products.
