Optimization of determination methods of astragaloside Ⅳ and analysis of contents of astragaloside Ⅳ in different origin and grade.
10.19540/j.cnki.cjcmm.20190412.201
- Author:
Qing-Wei CAO
1
;
Juan DU
2
;
Jin-Ping LUO
2
;
Rui ZHANG
1
;
Ke LI
3
;
Ai-Ping LI
3
;
Yue-Tao LIU
3
;
Xue-Mei QIN
3
Author Information
1. Modern Research Center for Traditional Chinese Medicine,Shanxi University Taiyuan 030006,China College of Chemistry and Chemical Engineering,Shanxi University Taiyuan 030006,China Key Laboratory of Effective Substances Research and Utilization in Traditional Chinese Medicine of Shanxi Province,Shanxi University Taiyuan 030006,China Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education,Shanxi University Taiyuan 030006,China.
2. Shanxi Provincial Institute for Food and Drug Control Taiyuan 030006,China.
3. Modern Research Center for Traditional Chinese Medicine,Shanxi University Taiyuan 030006,China Key Laboratory of Effective Substances Research and Utilization in Traditional Chinese Medicine of Shanxi Province,Shanxi University Taiyuan 030006,China Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education,Shanxi University Taiyuan 030006,China.
- Publication Type:Journal Article
- Keywords:
astragaloside Ⅳ;
content determination;
method optimization;
quality grade standard
- MeSH:
Astragalus Plant;
chemistry;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
analysis;
Saponins;
analysis;
Triterpenes;
analysis
- From:
China Journal of Chinese Materia Medica
2019;44(13):2813-2819
- CountryChina
- Language:Chinese
-
Abstract:
A rapid and accurate method for determination of astragaloside Ⅳ was established,which was further applied to determine the contents of astragaloside Ⅳ in 87 batches of different origin and different grade of Astragali Radix. The ROC curve was used to analyze the contents of astragaloside Ⅳ in different origin. Simultaneous contents of astragaloside Ⅳ in different grade were compared with chemometrics. HPLC-ELSD method was used to determine the contents of astragaloside Ⅳ. A Vensil MP C18 column( 4. 6 mm×250 mm,5 μm) was used with acetonitrile-water( 32 ∶68) as the mobile phase at a flow rateof 1 m L·min-1. The column temperature was 25 ℃ with ELSD parameters as follows: gas flow rate was 2. 5 L·min-1,the drift tube heating temperature was set to 105 ℃,and the gain value was 4. 0. The optimized method avoided the problem that the consumable quality unstable and the recovery rate was not high. The contents determined by the optimized method were higher than the pharmacopoeia method,with less time and high recovery rate. The ROC curve analysis showed that there was no significant difference of contents of astragaloside Ⅳ between the top grade of Shanxi wild-simulated Astragali Radix top and the first grade of Gansu cultivated Astragali Radix. The contents of astragaloside Ⅳ in the second,third and fourth grade of Shanxi wild-simulated Astragali Radix was significantly higher than those of produced from Gansu.There was a significant negative correlation between the contents of astragaloside Ⅳ and grade in Shanxi Astragali Radix. While there was no correlation for Gansu Astragali Radix. This study provided the basis for the quality grade standard of Astragali Radix.
