Effects of emodin on lipid accumulation and inflammation in hepatocytes.
10.19540/j.cnki.cjcmm.20190321.401
- Author:
Yin-Huan ZHANG
1
;
Xiao-Wei YANG
2
;
Yi-Hang DAI
1
;
Hong-Bin XIAO
3
Author Information
1. School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 100029,China.
2. Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
3. Research Center for Chinese Medicine Analysis and Transformation,Beijing University of Chinese Medicine Beijing 100029,China.
- Publication Type:Journal Article
- Keywords:
emodin;
inflammatory factor;
lipid accumulation;
liver injury
- MeSH:
Apolipoprotein B-100;
metabolism;
Cells, Cultured;
Emodin;
pharmacology;
Fatty Acid Synthase, Type I;
metabolism;
Hepatocytes;
drug effects;
metabolism;
Humans;
Inflammation;
Interleukin-6;
metabolism;
Lipid Metabolism;
Lipids;
NF-kappa B;
metabolism;
Sterol Regulatory Element Binding Protein 2;
metabolism
- From:
China Journal of Chinese Materia Medica
2019;44(13):2820-2826
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to explore the effect of emodin on lipid accumulation and inflammation in hepatocytes. The cell morphology was observed by microscopy. LDH release was detected by the kit. Levels of intracellular lipid droplets were observed by oil red O staining. The contents of TC and TG in cells were detected by the kit. Western blot was used to determine protein expressions of FASN,SREBF2,APOB,IL-6 and p-NF-κB in hepatocytes. The results showed that the levels of L02 cell LDH were significantly increased after being treated with emodin,and the cells showed shrinkage,volume reduction,decrease in quantity with the increase of dose. Red lipid droplets were observed in L02 hepatocytes. Intracellular TC and TG contents of L02 cell increased in a concentrationdependent manner,with significant differences between medium and high-dose groups( P < 0. 05). Protein expressions of FASN,SREBF2,IL-6 and p-NF-κB were significantly higher than those of the control group,and the expression level of APOB was significantly lower than that of the control group( P<0. 05). In conclusion,emodin could induce lipid accumulation and inflammatory damage in hepatocytes in a dose-dependent manner,which in turn could damage liver cells. This process was related to the up-regulation of FASN,SREBF2,IL-6,p-NF-κB,as well as the down-regulation of the protein expression of APOB.
