Screening of long non-coding RNA related to CYP450s involved in biosynthesis of tanshinones.
10.19540/j.cnki.cjcmm.20190324.101
- Author:
Yuan CAI
1
;
Ying MA
2
;
Juan GUO
2
;
Yong-Qing WANG
1
;
Qing DU
1
;
Tong CHEN
2
;
Shui-Han ZHANG
1
;
Lu-Qi HUANG
2
Author Information
1. Institute of Traditional Chinese Medicine,Hunan Academy of Traditional Chinese Medicine Changsha 410006,China.
2. State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
- Publication Type:Journal Article
- Keywords:
Salvia miltiorrhiza;
cytochrome P450;
long non-coding RNA;
tanshinone
- MeSH:
Cytochrome P-450 Enzyme System;
genetics;
Diterpenes, Abietane;
biosynthesis;
Gene Expression Regulation, Plant;
Plant Roots;
RNA, Long Noncoding;
genetics;
RNA, Plant;
genetics;
Salvia miltiorrhiza;
genetics
- From:
China Journal of Chinese Materia Medica
2019;44(12):2480-2485
- CountryChina
- Language:Chinese
-
Abstract:
Tanshinones are abietane-type norditerpenoid quinones that make up the main bioactive ingredients of traditional Chinese medicine Salvia miltiorrhiza. Cytochrome CYP450 plays an important role in the post-structural modification of tanshinone biosynthesis pathway. Long non-coding RNA( lncRNA) have been defined as transcripts longer than 200 nucleotides,which have been functionally characterized in regulating the growth and development,secondary metabolism and stress of medicinal plants. In this study,we perform a comprehensive identification of lncRNAs in response to tanshinone metabolism induced by yeast extract( YE) and Ag~+ S. miltiorrhiza hairy roots. Deep RNA sequencing was used to identify a set of different 8 942 lncRNAs,of which 6 755 were intergenic lncRNAs. We predicted a total of 1 115 814 lncRNA-coding gene pairs,including 122 lncRNA-coding gene as cis pairs. The correlation analysis between lncRNA and CYP450 related to tanshinone biosynthesis was carried out and a total of 16 249 lncRNA-CYP450 target gene pairs were identified. Further analysis with functional known CYP76 AH1,CYP76 AH3 and CYP76 AK1 involved in tanshinone biosynthesis,we also identified a set of 216 target genes. These candidate genes will be the important target in the downstream regulation mechanism analysis of the tanshinone biosynthesis pathway.
