Chemical profiling for bile acid derivatives in yak bile.
10.19540/j.cnki.cjcmm.20190321.202
- Author:
Yan CAO
1
;
Qing-Qing SONG
1
;
Jun LI
1
;
Shuang-Bing DENG
2
;
Hai-Jun QI
2
;
Peng-Fei TU
1
;
Yue-Lin SONG
1
Author Information
1. Modern Research Center for Traditional Chinese Medicine,School of Chinese Meteria Medica,Beijing University of Chinese Medicine Beijing 100029,China.
2. State Key Laboratory of Innovative Natural Medicine and Traditional Chinese Medicine Injections,Jiangxi Qingfeng Pharmaceutical Co.,Ltd. Ganzhou 341000,China.
- Publication Type:Journal Article
- Keywords:
bile acids;
isomers;
relative response-collision energy curve;
yak bile
- MeSH:
Animals;
Bile;
chemistry;
Bile Acids and Salts;
chemistry;
Cattle;
Chromatography, High Pressure Liquid;
Mass Spectrometry;
Taurine
- From:
China Journal of Chinese Materia Medica
2019;44(12):2538-2543
- CountryChina
- Language:Chinese
-
Abstract:
Bile acids( BAs),the major constituents of bile,are also known to be potential biomarkers of various diseases,especially liver disease. The systematic analysis of BAs is believed to be of great importance towards the clarification of the effective material basis for bile-type medicines,and the diagnosis and therapy of related diseases as well. As a part of systematic study on bile-type medicine ongoing in our group,this study lays emphasis on the isomer discrimination,and the improvement of analytical method of BAs. Further,this method was subsequently applied to elucidate in depth the chemical profile of BAs in yak bile. Regarding isomer discrimination for BAs,we constructed relative response-collision energy curves( RRCECs) by high performance liquid chromatographyion trap-time of flight-mass spectrometry( HPLC-IT-TOF-MS) in combination with high performance liquid chromatography-triple quadrupole-linear ion trap mass spectrometry( HPLC-Qtrap-MS). As a result,both the optimum collision energy( OCE) and CE_(50) exhibited great correlations with structural characteristics,thus enabling the isomer distinguishing,such as unconjugated BAs,glycine-conjugated BAs,and taurine-conjugated BAs. According to information provided by mass spectrometry,the comparison of OCE and CE_(50),retention time matching,combined with reference substances and database retrieval,a total of 30 bile acid derivatives were observed and identified in yak bile. The newly developed method could serve as a feasible tool for the in-depth characterization of BAs in bile and biological samples.
