Difference of alkaloid components between old stems and tender stems of Gelsemium elegans.
10.19540/j.cnki.cjcmm.20190416.201
- Author:
Jie WANG
1
;
Jie ZHANG
2
;
Chun-Ni ZHANG
2
;
Xiao-Chun LIAO
3
;
Jin LI
4
;
Bai-Ping MA
1
Author Information
1. Tianjin University of Traditional Chinese Medicine Tianjin 300193,China Institute of Radiation Medicine,Academy of Military Medical Sciences,Academy of Military Sciences Beijing 100850,China.
2. Institute of Radiation Medicine,Academy of Military Medical Sciences,Academy of Military Sciences Beijing 100850,China.
3. Yunnan Mingjinghengli Pharmaceutical Co.,Ltd. Kunming 650100,China.
4. Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Academy of Military Sciences Beijing 100850,China.
- Publication Type:Journal Article
- Keywords:
Gelsemium elegans;
alkaloids;
difference;
old stems;
tender stems
- MeSH:
Alkaloids;
analysis;
Chromatography, High Pressure Liquid;
Gelsemium;
chemistry;
Mass Spectrometry;
Plant Extracts;
Plant Stems;
chemistry
- From:
China Journal of Chinese Materia Medica
2019;44(12):2552-2558
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed to comprehensively assess the difference of alkaloid components between old stems and tender stems of Gelsemium elegans by using ultra high-performance liquid chromatography coupled with photo-diode array and quadrupole time-of-flight mass spectrometry( UPLC-Q-TOF/MS~E) and high-performance liquid chromatography coupled with UV detector( HPLC-UV). Firstly,the different components in old stems and tender stems were analyzed by UHPLC-Q-TOF/MSEcombined with principal component analysis( PCA) and orthogonal partial least squares discriminant analysis( OPLS-DA),respectively. As a result,17 major different components were found. At the same time,the distribution of these alkaloids in old stems and tender stems was determined,and the alkaloids with higher polarity are relatively higher in the tender stems,while the old stems are in the opposite case. In addition,three main components in the G. elegans were quantified by HPLC-UV. The results showed that the contents of koumine and humantenmine in old stems were higher than those in tender stems,and the content of gelsemine in tender stems was relatively high. This study systematically evaluated the differences of alkaloids between the old stems and tender stems of G. elegans,and quantified the main three alkaloids. It laid the foundation of the safe and effective application of G. elegans.
