Study on pharmacodynamic material basis of Naomaitong to protect neuronal cells based on PK-PD model.
10.19540/j.cnki.cjcmm.20190313.002
- Author:
Lan-Fang TAN
1
;
Chao CHEN
1
;
Xu-Lei FAN
1
;
Yu DONG
2
;
Yue-Wei GE
1
;
Shu-Mei WANG
1
Author Information
1. Guangdong Pharmaceutical University Guangzhou 510006,China Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica,State Administration of Traditional Chinese Medicine Guangzhou 510006,China Chinese Medicine Quality Engineering Technology Research Center of Guangdong Province/Higher-Education Guangzhou 510006,China.
2. Inner Mongolia Medical University Huhehot 010110,China.
- Publication Type:Journal Article
- Keywords:
Naomaitong;
PK-PD;
SVM;
cerebral apoplexy;
pharmacodynamic material basis
- MeSH:
Animals;
Drugs, Chinese Herbal;
pharmacology;
Neurons;
cytology;
Neuroprotective Agents;
pharmacology;
PC12 Cells;
Rats
- From:
China Journal of Chinese Materia Medica
2019;44(12):2588-2593
- CountryChina
- Language:Chinese
-
Abstract:
The PK-PD correlation models by using pharmacodynamics and pharmacokinetics were applied to study the material basis of Naomaitong,a clinical empirical prescription for the treatment of cerebral apoplexy,in inhibiting the death of PC12 nerve cells induced by Na_2S_2O_4 and Glu. In this experiment,PC12 cell death models induced by Na_2S_2O_4 and Glu were established respectively.With LDH lateral leakage and NO content as pharmacodynamic indexes,PK-PD model was established by SVM algorithm to evaluate the effective components of Naomaitong in inhibiting neural cell death. The results showed that the positive correlation of emodin methyl ether-8-O-β-D-glucopyranoside,aloe emodin,chrysophanol,rhein,emodin,ginsenoside Rg1,ginsenoside Rc,3'-methoxypuerarin and ligustilide was significant,obviously improving the LDH release and NO content. The results indicated that the contribution of Radix Puerariae Lobatae Radix and Rhei Radix et Rhizoma in Naomaitong could protect the nerve cell death induced by Na_2S_2O_4 and Glu respectively. PK-PD model was used to screen the neuroprotective components in Naomaitong,revealing the possible pharmacodynamic material basis of Naomaitong in the treatment of cerebral ischemia injury.
