Modulation effect of Acanthopanax senticosus polysaccharides through inflammatory cytokines in protecting immunological liver-injured mice.
10.19540/j.cnki.cjcmm.20190129.001
- Author:
Na ZHANG
1
;
Liang-You ZHAO
1
;
Di MAO
1
;
Zhao-Yuan DU
2
;
Xiao-Juan ZHANG
2
;
Xu-Nan ZHAI
2
;
Bai-Song AN
1
;
Shu-Min LIU
1
Author Information
1. Drug Safety Evaluation Center,Heilongjiang University of Chinese Medicine Harbin 150040,China.
2. Pharmaceutical College,Heilongjiang University of Chinese Medicine Harbin 150040,China.
- Publication Type:Journal Article
- Keywords:
Acanthopanax senticosus polysaccharides;
NF-κB;
concanavalin A;
immunological liver injury;
inflammatory cytokines
- MeSH:
Animals;
Chemical and Drug Induced Liver Injury;
drug therapy;
Cytokines;
metabolism;
Eleutherococcus;
chemistry;
Liver;
drug effects;
Mice;
Mice, Inbred BALB C;
NF-kappa B;
metabolism;
Peptides, Cyclic;
Polysaccharides;
pharmacology;
Random Allocation;
Signal Transduction
- From:
China Journal of Chinese Materia Medica
2019;44(14):2947-2952
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this paper was to discuss the protective effect and mechanism of Acanthopanax senticosus polysaccharides( ASPs) on immunological liver injury caused by conanavalin A( Con A). BALB/c mice were randomly divided into seven groups: control group,model group( Con A),low-,medium-,and high-dose( 36. 25,72. 5,145 mg·kg~(-1)) ASPs groups,bifendate( 200 mg·kg~(-1),positive drug) group and pyrrolidinedithiocarbamate( PDTC,NF-κB inhibitor,200 mg·kg~(-1)) group. ASPs groups and bifendate group were given with corresponding drugs by ig administration once daily for 7 d. Control group,model group and PDTC group were given with normal saline by ig administration once daily for 7 d. After the last ig administration,PDTC was given in DTC group by iv administration( 200 mg·kg~(-1)); 0. 5 h after that,Con A( 20 mg·kg~(-1)) was injected via the tail vein to induce immunological liver injury in all the mice except normal control group. The mice were killed 8 h later and their liver tissues were collected for histopathological examination. The contents of nitric oxide( NO),superoxide dismutase( SOD),malondialdehyde( MDA),reduced glutathione( GSHPX),interleukin( IL-1β) and tumor necrosis factor( TNF-α) in liver tissues were detected by kit assay. Western blot method was used to detect TNF-α,intercellular cell adhesion molecule-1( ICAM-1),inducible nitric oxide synthase( i NOS) and nuclear factor( NF-κB) protein expression in liver tissues. As compared with model group,ASPs not only could reduce the activity of MDA,NO,IL-1β and TNF-α,but also increase the content of GSH-PX and SOD; at the same time,the protein expression levels of TNF-α,ICAM-1,i NOS and NF-κB were reduced in liver tissues; in addition,inflammatory cell infiltration was alleviated,hepatocyte cytoplasm was loose and swollen,and nuclear condensation and staining were improved. ASPs has a protective effect on immunological liver injury,and the mechanism may be associated with regulating secretion of inflammatory cytokines and the expression of adhesion factor through NF-κB signaling pathway.
