Mechanism of Danzhi Jiangtang Capsules on improving liver injury in hyperlipidemia rats based on MAPK pathway.
10.19540/j.cnki.cjcmm.20190220.001
- Author:
Dong-Mei XU
1
;
Yong CHEN
1
;
Zhao-Hui FANG
2
;
Yun-Xia LU
1
Author Information
1. Anhui Medical University Hefei 230032,China.
2. the First Affiliated Hospital of Anhui University of Chinese Medicine Hefei 230031,China.
- Publication Type:Journal Article
- Keywords:
Danzhi Jiangtang Capsules;
MAPK pathways;
hyperlipidemia;
inflammation;
liver
- MeSH:
Animals;
Capsules;
Diet, High-Fat;
Drugs, Chinese Herbal;
pharmacology;
Hyperlipidemias;
drug therapy;
Inflammation;
Liver;
drug effects;
metabolism;
MAP Kinase Signaling System;
Male;
Rats;
Rats, Sprague-Dawley;
p38 Mitogen-Activated Protein Kinases;
metabolism
- From:
China Journal of Chinese Materia Medica
2019;44(14):2953-2959
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed to investigate the protective effect and preliminary mechanism of Danzhi Jiangtang Capsules( DJC) on liver of hyperlipidemic rats. The hyperlipidemia models were successfully made by high-fat diet for 12 weeks in male SD rats,and then divided into model control group and DJC treatment groups( 500 and 1 000 mg·kg~(-1)·d-1) via gavage administration for additional 8 weeks.The levels of serum lipid and liver metabolism indices were detected; HE and oil red O staining were used to observe the pathological changes of liver. Expression levels of extracellular regulated protein kinase 1/2( ERK1/2),c-Jun N-terminal kinase( JNK),and p38 mitogen-activated protein kinase( p38 MAPK) were detected by real-time polymerase chain reaction( RT-PCR). Expression of MCP-1,phosphorylated ERK( p-ERK),phosphorylated JNK( p-JNK),and phosphorylated p38 MAPK( p-p38) were analyzed by immunohistochemistry and Western blot. The results showed that DJC decreased body weight and serum levels of total cholesterol( TC),triglyceride( TG),alanine aminotransferase( ALT),aspartate aminotransferase( AST),increased serum high-density lipoprotein cholesterol( HDL-C) level,ameliorate injury and lipid deposition in the liver induced by the high-fat diet,decreased mRNA expression of ERK1/2,JNK and p-38 MAPK as well as protein expression of p-ERK,p-JNK,p-p38,and MCP-1,somewhat showing a dose-dependent effect. Therefore,DJC has an obvious protective effect on liver of hyperlipidemic rats with certain dose-dependent effect,and the mechanism may be related with inhibiting MAPK pathways and inflammation.
