Effects of saikosaponin b_2 on inflammation and energy metabolism in mice with acute liver injury induced by LPS/GalN.
10.19540/j.cnki.cjcmm.20190521.415
- Author:
Man YOU
1
;
Rui-Fang LI
1
;
Zi-Han GAO
1
;
Yuan-Ye LI
1
;
Wei-Yi LIU
1
;
Jian-Gang WANG
1
;
Hong-Wei WANG
2
;
San-Qiang LI
1
Author Information
1. Medical College,Henan University of Science and Technology Luoyang 471023,China.
2. School of Nursing,Henan University of Science and Technology Luoyang 471023,China.
- Publication Type:Journal Article
- Keywords:
NF-κB;
Sirt-6;
acute liver injury;
energy metabolism;
inflammation;
saikosaponin b2
- MeSH:
Animals;
Chemical and Drug Induced Liver Injury;
drug therapy;
Cytokines;
metabolism;
Energy Metabolism;
Galactosamine;
Inflammation;
drug therapy;
Lipopolysaccharides;
Liver;
drug effects;
Mice;
NF-kappa B;
metabolism;
Oleanolic Acid;
analogs & derivatives;
pharmacology;
Random Allocation;
Saponins;
pharmacology;
Sirtuins;
metabolism
- From:
China Journal of Chinese Materia Medica
2019;44(14):2966-2971
- CountryChina
- Language:Chinese
-
Abstract:
To study the effects of saikosaponin b2( SS-b2) on inflammatory factors and energy metabolism against lipopolysaccharide/galactosamine( LPS/Gal N) induced acute liver injury in mice. Mice were randomly divided into normal group( equal amount of normal saline),model group( 100 g·kg~(-1) LPS and 400 mg·kg~(-1) Gal N),low,medium,high dose group of SS-b2( SS-b25,10,20 mg·kg~(-1)·d-1) and positive control group( dexamethasone,10 mg·kg~(-1)). All of the groups except for the normal group were treated with LPS/Gal N though intraperitoneally injection to establish the acute liver injury model. The organ indexes were calculated. The levels of serum transaminases( ALT and AST) and the activities of ATPase( Na+-K+-ATPase,Ca2+-Mg2+-ATPase) in liver were detected. The activity of tumor necrosis factor-α( TNF-α),interleukin-1β( IL-1β) and interleukin-6( IL-6) were determined by the enzyme-linked immunosorbent assay( ELISA). The contents of lactate dehydrogenase( LDH) in liver were determined by micro-enzyme method. HE staining was used to observe the histopathological changes of the liver. Histochemical method was used to investigate the protein expression of liver lactate dehydrogenase-A( LDH-A). The protein expressions of Sirt-6 and NF-κB in the liver were detected by Western blot. According to the results,compared with the model group,there were significant changes in organ indexes in the high-dose group of SS-b2( P<0. 05). The level of ALT,AST,TNF-α,IL-1β,IL-6 and the activities of LDH in serum of mice with liver injury were significantly reduced in the medium and high dose groups of SS-b2( P<0. 01). With the increase of the concentration of SS-b2,the range of hepatic lesions and the damage in mice decreased. The activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase in liver of mice were significantly enhanced in each dose group( P<0. 01). The expression of NF-κB in liver tissues was significantly down-regulated in the medium and high dose group( P<0. 01). Meanwhile,the expression of Sirt-6 protein in the liver of mice with acute liver injury was significantly increased in each dose group( P<0. 01).In summary,SS-b2 has a significant protective effect on LPS/Gal N-induced acute liver injury in mice,which may be related to the down-regulation of NF-κB protein expression and up-regulation of Sirt-6 protein expression to improve inflammatory injury and energy metabolism.
