Study of mycelial polysaccharide from Paraisaria dubia of Ophiocordyceps gracilis asexual.
10.19540/j.cnki.cjcmm.20190318.201
- Author:
Yue WANG
1
;
Zhao-Lan LI
2
;
Fei-Ya SUO
1
;
Dong-Ping SUN
3
Author Information
1. College of Life Science and Technology,Xinjiang University Urumqi 830046,China.
2. College of Life Science,Nanjing University Nanjing 210093,China.
3. School of Chemistry Engineering,Nanjing University of Science and Technology Nanjing 210094,China.
- Publication Type:Journal Article
- Keywords:
Paraisaria dubia;
intracellular polysaccharide;
physicochemical properties
- MeSH:
Fungal Polysaccharides;
chemistry;
Galactose;
Glucose;
Hypocreales;
chemistry;
Mannose;
Mycelium;
chemistry
- From:
China Journal of Chinese Materia Medica
2019;44(8):1704-1709
- CountryChina
- Language:Chinese
-
Abstract:
In order to provide a foundation for the development and application of Ophiocordyceps gracilis and increase the new resources of cordyceps,an asexual Paraisaria dubia was isolated from an O. gracilis fruit body. After 10 days of liquid fermentation,white globular mycelium and clear transparent fermentation were produced. The mycelium was extracted by hot water and precipitated with ethanol to obtain intracellular crude polysaccharide. The protein was deproteinized to obtain deproteinized polysaccharide. The intracellular pure polysaccharide was purified by Sepharose 4 B column chromatography and were analyzed by UV,IR,1 H-NMR,and13 CNMR data,as well as GC and HPLC. The results showed that the intracellular polysaccharide of P. dubia was composed of glucose,galactose and mannose with a molar ratio of 25. 54 ∶2 ∶1. It was a β-configuration glycosylic bond,containing pyranoside. The initial connection of polysaccharide was β(1→2)(1→4)(1→6) connection. This experiment provides a theoretical basis for the development and application of P. dubia.
