Inhibitory effect of endophytic fungi from Dysosma versipellis on HIV-1 IN-LEDGF/p75 interaction.
10.19540/j.cnki.cjcmm.20190301.014
- Author:
Ya-Qin ZHOU
1
;
Da-Wei ZHANG
2
;
Li-Ying YU
3
;
Ying WEI
3
;
Hong-Zhen TANG
4
;
Shi-Ling YANG
4
;
Xiao-Ming TAN
4
Author Information
1. Guangxi University of Chinese Medicine Nanning 530200,China Guangxi Botanical Garden of Medicinal Plant Nanning 530023,China.
2. Institude of Bioinformatics and Medical Engineering,Jiangsu University of Technology Changzhou 213001,China.
3. Guangxi Botanical Garden of Medicinal Plant Nanning 530023,China.
4. Guangxi University of Chinese Medicine Nanning 530200,China.
- Publication Type:Journal Article
- Keywords:
Dysosma versipellis;
HIV-1;
LEDGF/p75;
endophytic fungi;
integrase;
protein-protein interaction
- MeSH:
Berberidaceae;
microbiology;
Endophytes;
Fungi;
chemistry;
HIV Integrase;
metabolism;
HIV-1;
drug effects;
Humans;
Intercellular Signaling Peptides and Proteins;
metabolism;
Protein Binding
- From:
China Journal of Chinese Materia Medica
2019;44(9):1808-1813
- CountryChina
- Language:Chinese
-
Abstract:
To determine the inhibitory effect of endophytic fungi from Dysosma versipellis on HIV-1 IN-LEDGF/p75 interaction,the protein-protein interaction between human immunodeficiency virus type 1( HIV-1) integrase and lens epithelial growth factor p75 protein( LEDGF/p75) was used as a target. The homogeneous time-resolved fluorescence( HTRF) technique was used in the inhibitory activity assay. The results showed that eight endophytic fungi with anti-IN-LEDGF/p75 interaction activity were screened out from fifty-three strains with different morphological characteristic. Among them,106 strain showed strong inhibitory activity against HIV-1 IN-LEDGF/p75 interaction with IC50 value of 5. 23 mg·L-1,and was identified as a potential novel species of Magnaporthaceae family by the analyses of ITS-rDNA,LSU and RPB2 sequences data. This study demonstrated that potential natural active ingredients against the HIV-1 IN-LEDGF/p75 interaction exist in the endophytic fungi of D. versipellis. These results may provide available candidate strain resources for the research and development of new anti-acquired immunodeficiency syndrome drugs.
