Preliminary study on antibacterial activity of artemisinin and its derivatives.
10.19540/j.cnki.cjcmm.20190131.001
- Author:
Mei HUANG
1
;
Jian-Ying SHEN
2
;
Cheng-Cheng DU
1
;
Ting YIN
2
;
Fang-Ji GE
2
;
Yu-Qing TAN
2
;
Jun LUO
3
Author Information
1. School of Basic Medical Sciences,Guizhou Medical University Guiyang 550000,China Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
2. Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
3. School of Basic Medical Sciences,Guizhou Medical University Guiyang 550000,China.
- Publication Type:Journal Article
- Keywords:
antibacterial test;
artemisinin;
artesunate;
broth dilution method;
dihydroartemisinin;
plate punching method
- MeSH:
Anti-Bacterial Agents;
pharmacology;
Artemisinins;
pharmacology;
Artesunate;
pharmacology;
Escherichia coli;
drug effects;
Microbial Sensitivity Tests;
Staphylococcus aureus;
drug effects
- From:
China Journal of Chinese Materia Medica
2019;44(9):1946-1952
- CountryChina
- Language:Chinese
-
Abstract:
In this study,in order to detect the antimicrobial activity of artemisinin and its derivatives artesunate and dihydroartemisinin,two methods including broth dilution and plate punching method were used to detect the antibacterial activity against gram-negative bacteria(Escherichia coli)and gram-positive bacteria(Staphylococcus aureus)of artemisinin,dihydroartemisinin and artesunate at various concentrations within 5 mmol·L~(-1)and at four time points(8,16,24,32 h).Two antibacterial positive drugs,streptomycin against E.coli and penicillin against S.aureus,were used as positive controls.Plate punching method showed that,unlike the results of 5 mmol·L~(-1)dihydroartemisinin or artesunate,no inhibition zone was detected at the same concentration of artemisinin after 24 h-treatment against E.coli.Broth dilution method showed that,the antibacterial activity of dihydroartemisinin against E.coli.was stronger than those of both artesunate and artemisinin;IC_(50)at24 h-treatment was 155.9μmol·L~(-1)for dihydroartemisinin,370.0μmol·L~(-1)for artesunate and none for artemisinin.Interestingly,dihydroartemisinin and artesunate showed the strongest antibacterial activity between 16-24 h,while artemisinin showed relatively stronger antibacterial activity between 8-16 h.Dihydroartermisinin showed no antibacterial activity against S.aureus.Above all,the antibacterial activity of artemisinins against E.coli is dihydroartemisinin>artesunate>artemisinin.Artemisinin and its derivatives have showed different antibacterial kinetics,and no antibacterial activity against S.aureus.has been detected with dihydroartemisinin.
