Rapid identification of Cordyceps and its products using DNA barcoding method.
10.19540/j.cnki.cjcmm.20190131.002
- Author:
Yan-Yan SU
1
;
Wen-Jia LI
2
;
Li YANG
1
;
Dan-Dan DING
1
;
Li XIANG
1
Author Information
1. Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
2. Key Laboratory of State Administration of Traditional Chinese Medicine,Sunshine Lake Pharma Co.,LTD Dongguan 523850,China.
- Publication Type:Journal Article
- Keywords:
COⅠ;
Cordyceps sinensis;
DNA barcoding;
ITS;
hosts;
identification
- MeSH:
Cordyceps;
classification;
DNA Barcoding, Taxonomic;
DNA Primers;
Mycelium;
Polymerase Chain Reaction
- From:
China Journal of Chinese Materia Medica
2019;44(10):1965-1973
- CountryChina
- Language:Chinese
-
Abstract:
Cordyceps is one of the most valuable traditional Chinese medicines. There are various counterfeits in markets because of high price and limited output. In this study,116 Cordyceps,146 hosts and 29 related products were collected and detected by using normal DNA barcoding technology and specific PCR method. The results indicated that Cordyceps and its adulterants could be distinguished from each other through DNA barcoding technology based on ITS and COⅠsequences. Two pairs specific primers ITSSF1/ITSSR1 and ITSSF2/ITSSR2 were developed to amplify 297 bp and 136 bp ITS regions of Cordyceps sinensis,respectively. It could be used to identify C. sinensis specifically and rapidly. Furthermore,specific primers ITSSF1/ITSSR1 and ITSSF2/ITSSR2 combined with ITS and COⅠsequences could differentiate powder Cordyceps from fermentation mycelia and could identify related products. Therefore,the method developed from this study could be applied to identify the powder of Cordyceps from fermentation mycelia and related products efficiently.
