Effects of different drying conditions on protein in Cordyceps.
10.19540/j.cnki.cjcmm.20181101.002
- Author:
Zheng-Ming QIAN
1
;
Jiao-Jiao FAN
2
;
Chun-Hong LI
1
;
Feng-Qing YANG
2
;
Wen-Jia LI
1
Author Information
1. State Key Laboratory of the State Administration of Traditional Chinese Medicine,Sunshine Lake Pharma Co.,LTD Dongguan 523850,China.
2. School of Chemistry and Chemical Engineering,Chongqing University Chongqing 401331,China.
- Publication Type:Journal Article
- Keywords:
2-DE;
Cordyceps;
SDS-PAGE;
protein
- MeSH:
Cordyceps;
chemistry;
Desiccation;
methods;
Electrophoresis, Gel, Two-Dimensional;
Electrophoresis, Polyacrylamide Gel;
Fungal Proteins;
analysis;
Molecular Weight
- From:
China Journal of Chinese Materia Medica
2019;44(10):1983-1988
- CountryChina
- Language:Chinese
-
Abstract:
In this study,the protein in different Cordyceps samples,which include fresh sample( S1),22 ℃ drying sample( S2),37 ℃ drying sample( S3) and 60 ℃ drying sample( S4),were analyzed by sodium dodecylsupinate-polyacrylamide gel electrophoresis( SDS-PAGE) and two-dimensional electrophoresis( 2-DE). The total protein contents in Cordyceps samples were from 1. 655-4. 493 mg·g~(-1) and the protein contents in fresh sample was the highest. The results of SDS-PAGE showed that the mainly ranges of protein molecular weight of Cordyces samples were 10-100 kDa and the numbers of protein bands were 28 to 41,the fresh sample had the maximum number of protein bands. The 2-DE profiles were analyzed by PDQuest software. The resulted indicated that 488-876 protein spots were detected in different Cordyceps samples and the isoelectric point( pI) was distributed between 4. 5 and 6. 5,the protein molecular weight was distributed in 10-20 kDa and 25-100 kDa,the fresh sample had the maximum number of protein spots. Therefore,the drying process could decrease contents and species of protein in Cordyceps,and the different drying conditions had different effects on protein. These results provide a reference for improving the drying process of Cordyceps.
