Cloning,subcellular localization and spatio-temporal expression analysis of a flavonoid 3-O-glucosyltransferase gene( SmUF3GT) in Salvia miltiorrhiza.
10.19540/j.cnki.cjcmm.20190301.009
- Author:
Hong-Yan LI
1
;
Jing-Ling LIU
1
;
Wei-Bo JIN
2
;
Zong-Suo LIANG
3
Author Information
1. College of Life Science,Northwest Agriculture and Forestry University Yangling 712100,China.
2. College of Life Science,Zhejiang Sci-Tech University Hangzhou 310018,China.
3. College of Life Science,Northwest Agriculture and Forestry University Yangling 712100,China Institute of Soil and Water Conservation,Chinese Academy of Sciences and the Ministry of Water Resources Yangling 712100,China College of Life Science,Zhejiang Sci-Tech University Hangzhou 310018,China.
- Publication Type:Journal Article
- Keywords:
Salvia miltiorrhiza;
Salvia miltiorrhiza f.alba;
SmUF3GT;
qRT-PCR;
subcellular localization;
temporal expression pattern
- MeSH:
Cloning, Molecular;
Flowers;
enzymology;
Gene Expression Regulation, Plant;
Glucosyltransferases;
genetics;
Open Reading Frames;
Plant Proteins;
genetics;
Salvia miltiorrhiza;
enzymology;
genetics
- From:
China Journal of Chinese Materia Medica
2019;44(10):2038-2045
- CountryChina
- Language:Chinese
-
Abstract:
The family of flavonoid 3-O-glucosyltransferase catalyzes the modification of anthocyanin from unstable-structure to stable-structure. In this study,based on homology cloning and transcriptome library,we isolated the full-length c DNA of UDP-glucose: flavonoid 3-O-glucosyltransferase( named SmUF3GT) from the flower tissues of S. miltiorrhiza. This gene was consisted of 1 353 bp open reading frames( ORF) encoding 450 amino acids. And the SmUF3GT protein was performed for the bioinformatic analysis. Our results showed that the protein was preliminary localized in the Golgi and peroxisome of cytosol,as well as plasma membrane and cell nuclear.QRT-PCR analyses indicated that SmUF3GT expressed differently in all tissues and organs but roots of S. miltiorrhiza and S. miltiorrhiza f.alba. During floral development,the expression of SmUF3GT showed a trend of rising fist and then down in purple-flower Danshen,whereas decreasing sharply fist and then slowly in white-flower Danshen. The present study provides basic information for further research on the network of synthesis and accumulation of flavonoids in S.miltiorrhiza.
