Isolation and identification of endophytic fungi producing harpagoside and harpagide from Scrophularia ningpoensis.
10.19540/j.cnki.cjcmm.20190301.008
- Author:
Zhan-Yun SHEN
1
;
Bo ZHU
2
;
Quan-Long ZHANG
2
;
Lu-Ping QIN
2
Author Information
1. College of Pharmaceutical Sciences,Zhejiang Chinese Medical University Hangzhou 311402,China College of Traditional Chinese Medicine,Zhejiang Pharmaceutical College Ningbo 315100,China.
2. College of Pharmaceutical Sciences,Zhejiang Chinese Medical University Hangzhou 311402,China.
- Publication Type:Journal Article
- Keywords:
Scrophularia ningpoensis;
endophytic fungi;
harpagide;
harpagoside
- MeSH:
China;
DNA, Fungal;
genetics;
DNA, Ribosomal Spacer;
genetics;
Endophytes;
classification;
metabolism;
Fungi;
classification;
metabolism;
Glycosides;
biosynthesis;
Iridoid Glycosides;
metabolism;
Pyrans;
metabolism;
Scrophularia;
microbiology
- From:
China Journal of Chinese Materia Medica
2019;44(10):2046-2050
- CountryChina
- Language:Chinese
-
Abstract:
The endophytic fungi from root,main stem,branch and leaf of Scrophularia ningpoensis were isolated from Zhejiang,whether these strains could yield harpagide or harpagoside were tested by HPLC and LC-MS. According to the morphological characteristic and the similarity of the nucleotide sequence of internal transcribed spacer( ITS) between r DNAs,the strains producing harpagide or harpagoside were identified. The results showed that 210 strains were isolated from the samples,which were classified into 9 orders,13 families and 17 genera by morphological study. Harpagide was detected in endogenous fungi ZJ17 and harpagoside was detected in endogenous fungi ZJ25 by HPLC coupled with LC-MS. ZJ17 was identified as Alternaria alternate and ZJ25 was identified as A.gaisen by its morphology and authenticated by ITS( ITS4 and ITS5 regions and the intervening 5. 8 S rDNA region).
