UPLC-MS~n analysis and certification evaluation of main chemical composition in naphthopyrone reference extract of Cassiae Semen.
10.19540/j.cnki.cjcmm.20190222.008
- Author:
Lu WANG
1
;
De-Yong ZHOU
1
;
Li-Juan JIANG
1
;
Jin-Hua ZHANG
1
;
Wei ZHANG
1
;
Yan-Yan JIANG
2
;
Bin LIU
1
Author Information
1. School of Chinese Pharmacy,Beijng University of Chinese Medicine Beijing 102488,China.
2. School of Chinese Pharmacy,Beijng University of Chinese Medicine Beijing 102488,China Quality Control Technology and Engineering Center of Chinese Medicine,Beijing Municipal Commission of Education Beijing 102488,China.
- Publication Type:Journal Article
- Keywords:
Cassiae Semen;
determination;
naphthopyrone;
reference extract;
uncertainty
- MeSH:
Cassia;
chemistry;
Certification;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
standards;
Mass Spectrometry;
Plant Extracts;
standards;
Quality Control;
Seeds;
chemistry
- From:
China Journal of Chinese Materia Medica
2019;44(10):2102-2109
- CountryChina
- Language:Chinese
-
Abstract:
The main chemical constituents of naphthopyrone reference extract( NRE) with definite content and relatively fixed chemical composition were analyzed and determined. Ultra-high performance liquid chromatography-LTQ-Orbitrap XL mass spectrometry and high performance liquid chromatography were used to systematically study NRE from the aspects of main chemical components and determination. The results showed that the chemical composition of naphthopyrone reference extract of Cassiae Semen was relatively fixed,and seven naphthalopyranones were identified. Cassiaside B_2,cassiaside C_2,rubrofusarin-6-O-β-D-gentiobioside and cassiaside C were the main chemical constituents of NRE,of which the determination and uncertainty results were( 11. 40+ 0. 26) %,( 11. 68+0. 24) %,( 16. 60+0. 22) %,( 28. 8+0. 48) %,respectively. This study contributed to the accurate evaluation of NRE and the foundation for the application of NRE in the quality control of Cassiae Semen,and provided a new idea for the replacement of single chemical reference substance by the reference extract of traditional Chinese medicine.
