Study on active components of Fufang Huangbai Ye for diabetic foot treatment by UPLC-LTQ-Orbitrap-MS and network pharmacology.
10.19540/j.cnki.cjcmm.20190328.201
- Author:
Xin LI
1
;
Huan-Huan WANG
2
;
Jing XU
1
;
Li-Ying TANG
1
;
Deng-Feng LI
1
;
Yi ZHANG
1
;
Qiang JIA
3
;
Hong-Jun YANG
1
;
Hong-Wei WU
1
;
Jing-Jing ZHANG
1
Author Information
1. Institute of Chinese Materia Medica,Chinese Academy of Chinese Medical Science Beijing 100700,China.
2. Institute of Chinese Materia Medica,Chinese Academy of Chinese Medical Science Beijing 100700,China Tianjin University of Traditional Chinese Medicine Tianjin 301617,China.
3. Shangdong Hanfang Pharmaceutical Co.,Ltd. Ji'nan 250000,China.
- Publication Type:Journal Article
- Keywords:
Fufang Huangbai Ye;
UPLC-LTQ-Orbitrap-MS;
active compounds;
diabetic foot;
network pharmacology
- MeSH:
Chromatography, High Pressure Liquid;
Diabetic Foot;
drug therapy;
Drugs, Chinese Herbal;
pharmacology;
Humans;
Mass Spectrometry;
Molecular Docking Simulation;
Phytochemicals;
isolation & purification;
pharmacology
- From:
China Journal of Chinese Materia Medica
2019;44(10):2110-2117
- CountryChina
- Language:Chinese
-
Abstract:
Chemical constituents of the Fufang Huangbai Ye( FFHB) were analyzed and identified by UPLC-ESI-LTQ-OrbitrapMS. The analysis was performed on an Waters HSS T3 reverse phase column( 2. 1 mm×100 mm,1. 8 μm). The mobile phase consisting of 0. 1% aqueous formic acid( A) and acetonitrile( B) was used with gradient elution,and the flow rate was 0. 3 mL·min~(-1).Based on the information of the accurate mass,the multistage fragment ions,the mass spectrometric data of the standard substance and the relative reference literature,the structure of the chemical constituents in FFHB were identified. Based on the identified compounds,network pharmacology study,including target prediction,functional enrichment,and molecular docking was applied to screen out the main active substances for treatment of diabetes foot and explore the potential mechanism. The results showed that a total of 138 compounds were identified,including 28 alkaloids,16 flavonoids,11 phenylethanoid glycosides,9 cycloolefins,11 cyclohexylethanol derivatives,28 phenolic acids and derivatives,3 lignans,4 terpenes,28 volatile oils and the others. Further,36 active substances for diabetes foot were screened out,and the functional enrichment showed the potential mechanism of FFHB were mainly seven functional items including inflammatory response,growth factor activity. This study combining the UPLC-LTQ-Orbitrap-MS technology and the network pharmacology provide a useful reference and basis for active compounds,quality control markers and the pharmacological mechanism of FFHB for diabetic foot treatment.
