Study on blood enrichment mechanism of steamed notoginseng based on metabolomics method.
10.19540/j.cnki.cjcmm.20190115.001
- Author:
Yin ZHANG
1
;
Qian-Qian FEI
2
;
Jing WANG
2
;
Fen-Xia ZHU
2
;
Yan CHEN
2
;
Dao-Quan TANG
3
;
Bin CHEN
2
Author Information
1. Jiangsu Provincial Key Laboratory for New Drug Research and Clinical Pharmacy,Xuzhou Medical University Xuzhou 221004,China Key Laboratory for Traditional Chinese Medicine Drug Release System Under State Administration of Traditional Chinese Medicine,Jiangsu Province Academy of Traditional Chinese Medicine,the Third Clinical Medical College of Nanjing University of Chinese Medicine ( Affiliated Provincial Hospital of Integrated Traditional Chinese Medicine and Western Medicine) Nanjing 210028,China.
2. Key Laboratory for Traditional Chinese Medicine Drug Release System Under State Administration of Traditional Chinese Medicine,Jiangsu Province Academy of Traditional Chinese Medicine,the Third Clinical Medical College of Nanjing University of Chinese Medicine ( Affiliated Provincial Hospital of Integrated Traditional Chinese Medicine and Western Medicine) Nanjing 210028,China.
3. Jiangsu Provincial Key Laboratory for New Drug Research and Clinical Pharmacy,Xuzhou Medical University Xuzhou 221004,China.
- Publication Type:Journal Article
- Keywords:
blood deficiency;
metabolomics;
steamed notoginseng
- MeSH:
Anemia, Hemolytic;
drug therapy;
Animals;
Biomarkers;
Drugs, Chinese Herbal;
pharmacology;
Mass Spectrometry;
Metabolome;
Metabolomics;
Panax notoginseng;
chemistry;
Rats;
Steam
- From:
China Journal of Chinese Materia Medica
2019;44(10):2139-2148
- CountryChina
- Language:Chinese
-
Abstract:
In this paper,ultra performance liquid chromatography coupled with time-of-flight mass spectrometry( UPLC-Q-TOFMS) technique was used to study the effects of steamed notoginseng on endogenous markers in plasma of rats with hemolytic anemia induced by N-acetyl phenyl hydrazine( APH). The aim was to find out the potential biomarkers and possible blood enriching mechanism of steamed notoginseng on hemolytic anemia rats. In the experiment,steamed notoginseng medicine pair( steamed notoginseng-ginseng)and compound medicines( Sanqi Yangxue Capsules) were used respectively to intervene in APH-induced hemolytic anemia model rats.Then blood routine indexes such as red blood cells( RBC),hemoglobin( Hb) and related organ indexes were determined. As compared with the blank group,the RBC and Hb levels in the model group were substantially decreased( P< 0. 01),while the liver and spleen organ indexes were increased( P< 0. 05). The results of blood routine and organ index demonstrated that the blood deficiency model was successfully established. Steamed notoginseng can significantly increase the RBC level of rats( P<0. 01),and the related indicators of each drug group had a trend of returning to normal levels,verifying the blood enriching effect of steamed notoginseng. The UPLC-Q-TOF-MS technique,principal component analysis( PCA) and partial least squares-discrimination analysis( PLS-DA) were used to analyze the metabolic profiles between the normal group and the model group. Twenty-six potential biomarkers for hemolytic anemia were screened in plasma. Nine metabolites such as retinol,L-valine,and arachidonic acid were down-regulated in the blood deficiency rats,and 17 metabolites such as protoporphyrin Ⅸ and niacinamide were up-regulated. The metabolic level of biomarkers could be changed to a normal state after rats were given with steamed notoginseng,drug pairs,and compound prescriptions. It can be speculated that steamed notoginseng may play a role of blood tonifying by improving biosynthesis of valine,leucine and isoleucine,as well as metabolic pathways such as retinol metabolism and arachidonic acid metabolism.
