Study on compatibility of active ingredients of Simiao Yongan Decoction in rats with in situ single-pass intestinal perfusion model.
10.19540/j.cnki.cjcmm.20190119.001
- Author:
Xiao YU
1
;
Sen-Sen CHI
1
;
Qi-Shu JIAO
1
;
Li-Juan JIANG
1
;
Wei-Hua WANG
2
;
Yan-Yan JIANG
1
;
Bin LIU
1
Author Information
1. School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 102488,China.
2. National Institute of Metrology Beijing 100029,China.
- Publication Type:Journal Article
- Keywords:
Simao Yongan Decoction;
compound compatibility;
in situ single-pass intestinal perfusion model;
intestinal absorption kinetics
- MeSH:
Animals;
Drugs, Chinese Herbal;
pharmacokinetics;
Intestinal Absorption;
Intestines;
Phytochemicals;
pharmacokinetics;
Rats
- From:
China Journal of Chinese Materia Medica
2019;44(10):2163-2170
- CountryChina
- Language:Chinese
-
Abstract:
To study the compatibility rule of Simao Yongan Decoction,the rat single pass intestinal perfusion model in situ was used in this study. On the basis of early research,the five kinds of anti-inflammatory active ingredients,i.e. chlorogenic acid,liquiritin,hyperoside,angoroside C and isochlorogenic acid C in Simao Yongan Decoction were selected as research objects. The contents of the above five actives compounds with various compatibility combinations and in different intestinal segment perfusates were determined by using the method of ultra-performance liquid chromatography-mass spectrometry( UPLC-MSn). The kinetic parameters of intestinal absorption of the five anti-inflammatory active ingredients were calculated,which could be used to evaluate the intestinal absorption of each component in different combinations. The results showed that the absorption parameters of liquiritin in ileum were highest in Glycyrrhizae Radix et Rhizoma single herb,while the absorption parameters of other four components in ileum and duodenum were highest in the compatible combinations. Among them,the absorption parameters of chlorogenic acid in ileum and duodenum were highest in the whole prescription compatibility; ischlorogenic acid C showed higher absorption levels in the whole prescription and the herb compatibility of Lonicerae Japonicae Flos-Scrophulariae Radix-Glycyrrhizae Radix et Rhizoma. However,the absorption levels of hyperoside and angoroside C in different compatibilities were quite different in ileum and duodenum. In this study,the intestinal absorption of five anti-inflammatory active ingredients in Simiao Yongan Decoction with different compatibility combinations was investigated,revealing that the absorption of active ingredients varied with the different compatibility combinations and different intestinal segments. At the same time,the above research also indicated that the absorption of active ingredients could be obviously promoted by the compatibility of compound prescriptions,laying a foundation for the research on the compatibility rule of Simiao Yongan Detection from the biological point of view.
