Effects of exogenous IBA and fungal elicitor on growth of in vitro roots culture of Dysosma versipellis and production of podophyllotoxin.
10.19540/j.cnki.cjcmm.20190301.012
- Author:
Xiao-Ming TAN
1
;
Li-Ying YU
2
;
Hong-Zhen TANG
1
;
Li-Chun ZHAO
1
;
Ya-Qin ZHOU
3
Author Information
1. Guangxi University of Chinese Medicine Nanning 530200, China.
2. Guangxi Botanical Garden of Medicinal Plant Nanning 530023, China.
3. Guangxi University of Chinese Medicine Nanning 530200, China Guangxi Botanical Garden of Medicinal Plant Nanning 530023, China.
- Publication Type:Journal Article
- Keywords:
Dysosma versipellis;
HPLC;
IBA;
fungal elicitor;
in vitro roots;
podophyllotoxin
- MeSH:
Ascomycota;
Berberidaceae;
chemistry;
Endophytes;
Plant Roots;
drug effects;
growth & development;
Podophyllotoxin;
biosynthesis;
Tissue Culture Techniques
- From:
China Journal of Chinese Materia Medica
2019;44(11):2226-2230
- CountryChina
- Language:Chinese
-
Abstract:
Using the White as basic medium, the effects of the exogenous IBA and endophytic fungal elicitor on the growth of in vitro roots cultures of Dysosma versipellis and production of podophyllotoxin were investigated in this study. The results showed that the IBA and the endophytic fungus Zasmidium syzygii elicitor could increase the content of podophyllotoxin of in vitro roots of D. versipellis after 3 weeks. The White medium added with 3 mg·L~(-1) IBA induced the highest increase of podophyllotoxin(1 830.86 μg·g~(-1)), which was 2.07 folds greater than the control, and followed by 1.5 mg·L~(-1) IBA, fungal elicitor, 1 mg·L~(-1) IBA, 0.5 mg·L~(-1) IBA and 4.5 mg·L~(-1) IBA, which was 1.82, 1.71, 1.63, 1.43 and 1.1 folds greater than the control, respectively. The results also showed that the growth of roots was certain positively correlated with the change of IBA concentration. Therefore, 3 mg·L~(-1) IBA was the most suitable for the production of podophyllotoxin in the in vitro roots of D. versipellis, and the stimulating effect of Z. syzygii fungal elicitor was between 1.5 mg·L~(-1) and 1 mg·L~(-1) IBA, which was a potential natural elicitor to induce the accumulation of podophyllotoxin in future production.
