Preparation, characterization and dissolution characteristics of dragon's blood extract nanosuspensions.
10.19540/j.cnki.cjcmm.20181221.005
- Author:
Li-Fang WANG
1
;
Xiao-Nan CHEN
1
;
Jun LI
1
;
Peng-Fei TU
1
;
Jin-Ling WANG
1
Author Information
1. School of Chinese Materia Medica, Modern Research Center for Traditional Chinese Medicine,Beijing University of Chinese Medicine Beijing 100029, China.
- Publication Type:Journal Article
- Keywords:
dragon′s blood extract;
high pressure homogenization;
nanosuspensions;
single factor test;
stabilizer
- MeSH:
Calorimetry, Differential Scanning;
Nanoparticles;
Particle Size;
Plant Extracts;
chemistry;
Solubility;
Suspensions;
X-Ray Diffraction
- From:
China Journal of Chinese Materia Medica
2019;44(11):2236-2243
- CountryChina
- Language:Chinese
-
Abstract:
The single-factor test was used to optimize the high-pressure homogenization method to prepare the phenolic extract nanosuspensions(DBNs). The physicochemical properties of the obtained nanosuspensions were characterized and the cumulative release in vitro was evaluated. The results showed that the drug concentration was 0.5 g·L~(-1), the mass concentrations of PVPK30 and SDS were 0.5 and 0.25 g·L~(-1), respectively, the probe ultrasonic time was 5 min, the homogenization pressure was 900 bar, and the number of homogenization was 2 times. The prepared DBNs had an average particle size of(168.80±0.36) nm, polydispersity index(PDI) of 0.09±0.04, stability index(SI) of 0.85, and DBNs were stable for storage within 30 days. Scanning electron microscopy showed that the particle size of the dragon's blood extract was reduced and the uniformity was improved in the obtained nanosuspensions. X-ray diffraction pattern and differential scanning calorimetry showed that the phenolic extract of dragon's blood was still in an amorphous state after being prepared into nanosuspensions. The results of saturated solubility measurement showed that the solubility of DBNs lyophilized powder reached 6.25 g·L~(-1), while the solubility of DB raw powder was only 28.67 mg·L~(-1). The in vitro dissolution experiments showed that DBNs lyophilized powder accumulated in gastrointestinal fluid for 8 h. The release amount was 90%,the cumulative release of the raw powder in the gastrointestinal fluid for 24 h was less than 1%, and the solubility and dissolution rate of the DBNs lyophilized powder were significantly higher than the DB raw powder. The method is simple in process and convenient in operation, and can successfully prepare uniform and stable nanosuspensions to improve its solubility, and provides a research basis for solving the application limitation of dragon's blood extract.
