Chemical constituents from green walnut husks and their antitumor activity in vitro.
10.19540/j.cnki.cjcmm.20190321.201
- Author:
Dong-Xue SUN
1
;
Xiong-Fei GUO
2
;
La-Tengtuya A
2
;
Xiao-Ling MA
2
;
Hong-Yan WEI
2
;
Guo-Xu MA
2
;
Lei-Ling SHI
2
;
Jing ZHANG
3
Author Information
1. College of Chinese Medicine Material, Jilin Agricultural University Changchun 130118, China Xinjiang Institute of Chinese and Ethnic Medicine Urumqi 830002, China.
2. Xinjiang Institute of Chinese and Ethnic Medicine Urumqi 830002, China.
3. College of Chinese Medicine Material, Jilin Agricultural University Changchun 130118, China.
- Publication Type:Journal Article
- Keywords:
Juglandaceae;
antitumor activity;
chemical constituents;
green walnut husks
- MeSH:
Antineoplastic Agents, Phytogenic;
isolation & purification;
pharmacology;
Chromatography, High Pressure Liquid;
HT29 Cells;
HeLa Cells;
Humans;
Juglans;
chemistry;
Molecular Structure;
Phytochemicals;
isolation & purification;
pharmacology;
Plant Extracts;
chemistry
- From:
China Journal of Chinese Materia Medica
2019;44(11):2278-2282
- CountryChina
- Language:Chinese
-
Abstract:
Fourteen chemical constituents, including 5-hydroxy-4-methoxy-1-tetralone(1), 4,8-dihydroxy-1-tetralone(2), 4,5-dihydroxy-α-tetralone(3), blumenol B(4), dehydrovomifoliol(5), megastigm-5-ene-3,9-diol(6), juglanin B(7), blumenol C(8), loliolide(9), oleracone B(10), syringarsinol(11), pinoresinol(12), methyl 4-hydroxy-3-methoxybenzoate(13), and isovanillic acid(14), were isolated from the dichloromethane fraction of 95% methanol extract of green walnut husks by silica gel and MCI column chromatography, and Pre-HPLC. Their structures were determined by spectroscopic methods, such as NMR, MS and so on. Among them, compounds 1, 4-6, 8-13 were isolated from the green walnut husks for the first time, and compounds 4-6, 8, 10, 12, 13 were isolated from the Juglans genus for the first time. All of isolates were detected their inhibitory activities against HeLa, HGC-27 and Ht-29 cell lines by the MTT assay. The result showed that compounds 2, 3, 7, 9 and 11 exhibited inhibitory activity against the tested cell line. The IC_(50) of 7 were 26.5, 9.0, 25.4 μmol·L~(-1), respectively.
