Spectrum-effect relationships on enriching blood activities of fresh and dry roots of Angelica sinensis.
10.19540/j.cnki.cjcmm.20190321.211
- Author:
Jie-Li LIU
1
;
Ying ZHANG
2
;
Xia MA
2
;
Shu-Fang DENG
2
;
Dong-Mei XING
2
;
Yu-Jing SUN
1
;
Ya-Li WANG
3
Author Information
1. Gansu University of Chinese Medicine Lanzhou 730000, China Key Laboratory of Quality and Standard of Traditional Chinese Medicine in Gansu Province Lanzhou 730000, China.
2. Gansu University of Chinese Medicine Lanzhou 730000, China.
3. Gansu University of Chinese Medicine Lanzhou 730000, China Key Laboratory of Quality and Standard of Traditional Chinese Medicine in Gansu Province Lanzhou 730000, China the Research Insitute of Industrial Development of Angelica sinensis in Gansu Province Lanzhou 730000, China.
- Publication Type:Journal Article
- Keywords:
blood deficiency;
dry roots of A.sinensis;
enriching blood activities;
fresh roots of Angelica sinensis;
spectrum-effect relationship
- MeSH:
Angelica sinensis;
chemistry;
Animals;
Chromatography, Liquid;
Drugs, Chinese Herbal;
pharmacology;
Mass Spectrometry;
Phytochemicals;
pharmacology;
Plant Roots;
chemistry;
Rats
- From:
China Journal of Chinese Materia Medica
2019;44(11):2308-2316
- CountryChina
- Language:Chinese
-
Abstract:
Ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(LC-MS) was used to establish the chromatography fingerprint for fresh(FRAS) and dry(RAS) roots of Angelica sinensis from 10 different places. The rat model of blood deficiency was established by acetyl-phenyl-hydrazine(APH) and cyclophosphamide(CTX). Then grey relational analysis(GRA) and partial least squares regression(PLS) were used to investigate the spectrum-effect relationship between the relative contents and the data of enriching blood pharmacodynamics efficacy. The results showed that the FRAS and RAS had certain enriching blood activities(P<0.05). The contribution degree of the FRAS and RAS to enriching blood activities of each common peaks were determined by regression coefficient. Among them, 4 common peaks contributed significantly to the effect of enriching blood activities, P1(unknown), P2(unknown), P7(ferulic acid), and P11(senkyunolide A) respectively. This paper investigated the spectrum-effect relationship between enriching blood activities and LC-MS chromatography fingerprint of RAS and FRAS, and determined the effective compositions of RAS and FRAS with enriching blood activities. It lays a theoretical foundation for the comprehensive development and utilization of A. sinensis.
