Efficacy and mechanism of Lianhua Qingwen Capsules(LHQW) on chemotaxis of macrophages in acute lung injury (ALI) animal model.
10.19540/j.cnki.cjcmm.20190210.001
- Author:
Qi LI
1
;
Jie YIN
2
;
Qing-Sen RAN
1
;
Qing YANG
1
;
Li LIU
1
;
Zheng ZHAO
1
;
Yu-Jie LI
1
;
Ying CHEN
1
;
Li-Dong SUN
1
;
Ya-Jie WANG
1
;
Xiao-Gang WENG
1
;
Wei-Yan CAI
1
;
Xiao-Xin ZHU
1
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China School of Chinese Materia Medica, Capital Medical University Beijing 100069, China.
- Publication Type:Journal Article
- Keywords:
Lianhua Qingwen Capsules;
MCP-1;
acute lung injury;
macrophage
- MeSH:
Acute Lung Injury;
microbiology;
Animals;
Bacterial Infections;
drug therapy;
Bronchoalveolar Lavage Fluid;
Capsules;
Chemokine CCL2;
metabolism;
Chemotaxis;
Drugs, Chinese Herbal;
pharmacology;
Humans;
Lipopolysaccharides;
Lung;
Macrophages;
drug effects;
Mice;
RAW 264.7 Cells;
Random Allocation;
THP-1 Cells;
Tumor Necrosis Factor-alpha;
metabolism
- From:
China Journal of Chinese Materia Medica
2019;44(11):2317-2323
- CountryChina
- Language:Chinese
-
Abstract:
This paper was mainly to discuss the potential role and mechanism of Lianhua Qingwen Capsules(LHQW) in inhibiting pathological inflammation in the model of acute lung injury caused by bacterial infection. For in vitro study, the mRNA expression of MCP-1 in RAW264.7 cells and THP-1 cells, the content of MCP-1 in cell supernatant, as well as the effect of LHQW on chemotaxis of macrophages were detected. For in vivo study, mice were randomly divided into 7 groups, including normal group, model group(LPS 5 mg·kg~(-1)), LHQW 300, 600 and 1 200 mg·kg~(-1)(low, middle and high dose) groups, dexamethasone 5 mg·kg~(-1) group and penicillin-streptomycin group. Then, the anal temperature was detected two hours later. Dry weight and wet weight of lung tissues in mice were determined; TNF-α and MCP-1 levels in alveolar lavage fluid and MCP-1 in serum were detected. In addition, the infiltration of alveolar macrophages was also observed and the infiltration count of alveolar macrophages was measured by CCK-8 method. HE staining was also used to observe the inflammatory infiltration of lung tissues in mice. Both of the in vitro and in vivo data consistently have confirmed that: by down-regulating the expression of MCP-1, LHWQ could efficiently decrease the chemotaxis of monocytes toward the pulmonary infection foci, thus blocking the disease development in ALI animal model.
