Preventive and therapeutic effects of Keluoxin Capsules on early diabetic retinopathy in db/db mice.
10.19540/j.cnki.cjcmm.20190422.402
- Author:
Yun LUO
1
;
Shan LU
1
;
Li-Tao LIU
2
;
Ke XU
2
;
Man-Qian ZHAO
2
;
Liang YE
2
;
Quan WU
2
;
Chuan-Zhen TENG
2
;
Xiao KE
2
;
Gui-Bo SUN
1
;
Xiao-Bo SUN
1
Author Information
1. Institute of Medicinal Plant Development, Peking Union Medical College and Chinese Academy of Medical Sciences Beijing 100193, China.
2. Chengdu Kanghong Pharmaceuticals Group Chengdu 610036, China.
- Publication Type:Journal Article
- Keywords:
Keluoxin Capsules;
diabetic retinopathy;
retinal function;
retinal ganglion layer
- MeSH:
Animals;
Capsules;
Diabetes Mellitus, Experimental;
Diabetic Retinopathy;
drug therapy;
Fluorescein Angiography;
Hypoglycemic Agents;
pharmacology;
Male;
Mice;
Random Allocation;
Retina;
drug effects
- From:
China Journal of Chinese Materia Medica
2019;44(11):2324-2330
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this paper was to investigate the preventive effects of Keluoxin Capsules(KLX) on diabetic retinopathy in db/db mice. One hundred male db/db diabetic mice(45-55 g, 8 weeks) were randomly divided into 5 groups(model, KLX low dose, KLX middle dose, KLX high dose, Dobesilate) and 20 male C57 BL/KsJdb~(+/+) were taken as control group. Body weight and fasting blood-glucose were detected every week. Mice were administrated with saline(control and model group), KLX(780, 1 560, 3 120 mg·kg~(-1)·d~(-1), ig), Dobesilate(195 mg·kg~(-1)·d~(-1), ig) for 20 weeks, respectively. At the end of the administration, optical coherence tomography, fundus fluorescein angiography and electroretinogram of the retina were measured. The eyeball was extirpated and retina was isolated to make paraffin section, followed by HE staining and glial fibrillary acidic protein(GFAP) immunohistochemistry. The results indicated that KLX has no obvious effect on body weight and fasting blood level in db/db mice. However, KLX could significantly regulate the thickness of retinal ganglion layer and inner plexiform layer. KLX was able to remarkably reduce the quantity of diabetic microvessel. Meanwhile, KLX could notably improve retinal function. Moreover, KLX could observably modulate the cell arrangement and edema in each layer. There was no markable difference in retina according to the immunochemistry assay. In the present study, KLX exert marked preventive effects on diabetic retinopathy in db/db mice, which provided an experimental evidence for clinical use.
