Ginsenoside Rg_1 induces leukemia stem cell senescence via SIRT1/TSC_2 signal axis.
10.19540/j.cnki.cjcmm.20190218.002
- Author:
Yan-Long TANG
1
;
Yue ZHOU
2
;
Cheng-Gui ZHANG
2
;
Heng LIU
2
;
Ya-Ping WANG
3
;
Yuan LI
2
;
Yan-Jun HAN
2
;
Cui-Li WANG
2
Author Information
1. the First Affiliated Hospital of Dali University Dali 671000, China.
2. Department of Histology and Embryology, Dali University, Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D Dali 671000, China.
3. Laboratory of Stem Cell and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University Chongqing 400016, China.
- Publication Type:Journal Article
- Keywords:
CD34~+CD38~-LSCs;
SIRT1;
TSC_2;
ginsenoside Rg_1;
senescence
- MeSH:
Cellular Senescence;
drug effects;
Ginsenosides;
pharmacology;
Humans;
Leukemia, Myeloid, Acute;
Neoplastic Stem Cells;
drug effects;
Signal Transduction;
Sirtuin 1;
metabolism;
Tuberous Sclerosis Complex 2 Protein;
metabolism;
Tumor Cells, Cultured
- From:
China Journal of Chinese Materia Medica
2019;44(11):2348-2352
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this paper was to investigate the effect of SIRT1/TSC_2 signal axis on leukemia stem cell senescence induced by ginsenoside Rg_1. CD34~+CD38~- leukemia stem cells(CD34~+CD38~-LSCs) was isolated by magnetic cell sorting(MACS) and divided into two groups. The control group cells were routinely cultured, 40 μmol·L~(-1) ginsenoside Rg_1 was added to the control group for co-culture in Rg_1 group. The effect of Rg_l to induce CD34~+CD38~-LSCs senescence were evaluated by senescence-associated β-Galactosidase(SA-β-Gal) staining, cell cycle assay, CCK-8 and Colony-Assay. The expression of senescence associated SIRT1, TSC_2 mRNA and protein was examined by Real-time fluorescence quantitative PCR(FQ-PCR) and Western blot. The results showed that the CD34~+CD38~-LSCs could effectively be isolated by MACS, and the purity of CD34~+CD38~-LSCs is up to(95.86±3.04)%. Compared with the control group, the percentage of positive cells expressed SA-β-Gal in the Rg_1 group is increased, the senescence morphological changes were observed in the CD34~+CD38~-LSCs in the Rg_1 group. The proliferation inhibition rate and the number of cells entered G_0/G_1 phase in the Rg_1 group were increased, but the colony-formed ability was decreased, Rg_1 could significantly inhibit the proliferation and self-renewal ability of CD34~+CD38~-LSCs. The expression of SIRT1 and TSC_2 mRNA and protein were down regulated in the Rg_1 group compared with the control group. Our research implied that Rg_1 may induce the senescence of CD34~+CD38~-LSCs and SIRT1/TSC_2 signal axis plays a significant role in this process.
