Mechanism research of curcumin on cancer cells based on cell metabolic profiling.
10.19540/j.cnki.cjcmm.20190121.001
- Author:
Jia-Hui HU
1
;
Pan-Pan XU
1
;
Li-Juan HOU
2
;
Min LIU
1
;
Hua WANG
1
;
Guang-Hui GAO
3
;
Li-Xin SUN
1
Author Information
1. School of Pharmacy, Shenyang Pharmaceutical University Benxi 117004, China.
2. School of Traditional Chinese Medicine, Shenyang Pharmaceutical University Benxi 117004, China.
3. Liaoning Institute for Food and Control Shenyang 110015, China.
- Publication Type:Journal Article
- Keywords:
GC-MS;
anticancer;
cell metabolism;
curcumin;
metabolic pathway
- MeSH:
Antineoplastic Agents, Phytogenic;
pharmacology;
Cell Line, Tumor;
Curcumin;
pharmacology;
Gas Chromatography-Mass Spectrometry;
Humans;
Metabolic Networks and Pathways;
Metabolome;
Metabolomics;
Principal Component Analysis
- From:
China Journal of Chinese Materia Medica
2019;44(11):2359-2366
- CountryChina
- Language:Chinese
-
Abstract:
In this study, gas chromatography coupled with mass spectrometry(GC-MS) was used to analyze the changes of 12 kinds of cancer cells treated by curcumin. The related differential metabolites were screened and the metabolic pathways were analyzed to explore the anti-tumor mechanism of curcumin. Methyl thiazol tetrazolium(MTT) assay was used to detect the 50% inhibiting concentration(IC_(50)) of curcumin on 12 human tumor cells. After treatment with curcumin for 48 h, the cells were collected and analyzed by GC-MS, followed by pathway analysis and multivariate data analysis including principal component analysis(PCA), orthogonal partial least squares discriminant analysis(OPLS-DA) and One-way analysis of variance(ANOVA),etc. Overall, 34 metabolites showed significant concentration changes after intervention for 48 h, mainly involving multiple metabolic pathways, including lysine degradation, glycine, serine and threonine metabolism, arginine and proline metabolism, cysteine and methionine metabolism, aminoacyl-tRNA biosynthesis, primary bile acid biosynthesis, lysine biosynthesis. In this study, the anti-tumor mechanisms of curcumin interfering with energy metabolism, amino acid metabolism, microtubule system, protein synthesis and oxidative stress response of tumor cells were analyzed from the perspective of metabolism, providing a new reference for further tumor pharmacology study.
