Down-Regulation of MiR-125b Reverses Drug Resistance of Doxorubicin-Resistant Leukemia Cells.
10.7534/j.issn.1009-2137.2018.06.005
- Author:
Yi-Min DENG
1
;
Ai-Ling LUO
2
;
Guang-Feng LI
1
;
Man-Si CAI
2
;
Ling XU
2
;
Mu-Xia YAN
3
Author Information
1. Department of Emergency, The First People's Hospital of Guangzhou, Guangzhou 510180, Guangdong Province, China.
2. Institute of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623, Guangdong Province, China.
3. Institute of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623, Guangdong Province, China.E-mail: zichemama@126.com.
- Publication Type:Journal Article
- MeSH:
Down-Regulation;
Doxorubicin;
Drug Resistance, Neoplasm;
Humans;
K562 Cells;
Leukemia;
MicroRNAs
- From:
Journal of Experimental Hematology
2018;26(6):1610-1615
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate whether the down-regulation of miR-125b can reverse the drug-resistence of doxorubicine-resistant leukemia cell lines or not, so as to explore a new method for treatment of drug-resistant leukemia patients.
METHODS:The expression levels of miR125b in doxorubicine drug-sensitive and doxorubicine drug-resistant leukemia cell lines.HL-60, K562 and HL-60/Dox, the K562/Dox were detected by using RT-qPCR; the up-regulation or inhibition of miR-1256 expression in HL-60/Dox were performed by electroporation transfection, then the viability of cells treated with doxorubicine of different concentration was detected by CCK-8 method, the proliferation inhibition curve of cells was drawed, and the IC was calculated.
RESULTS:The miR-125b expression was obviously up-regulated in drug-resistant cell lines HL-60/DOX and K562/DOX, as compared with HL-60 and K562 cell lines. The miR-125b expression level in HL-60/DOX and K562/DOX cells was 15 times and 5 times higher than that in HL-60 and K562 cells, respectively. The up-regulating or inhibiting expression of miR-125b in HL-60/DOX cells found that the proliferation inhibition rate in cells transfected with miR-125b mimic significantly decreased, compared with control group (P<0.01), while the proliferation inhibition rate in cells transfected with miR-125b inhibitor significantly increased, compared with control group(P<0.01).
CONCLUSION:The miR-125b expression in HL-60/Dox and K562/Dox cells has been up-regulated, down-regulation of miR-125b expression can reverse the drug resistance of leukemia cells to doxorubicine.
