Effect of Quercetin on the Cell Cycle and Adhesion Molecules of NOD/SCID Mice with Acute B Lymphocytic Leukemia.
10.7534/j.issn.1009-2137.2018.06.006
- Author:
Li WANG
1
;
Hong-Wei DAI
2
;
Jun ZHENG
2
;
Jiao ZHOU
2
;
De-Sen CHEN
3
Author Information
1. Depertment of Hematology, The Affiliated People's Hospital of Hubei Medical and Pharmaceutical College, Shiyan 442000, Hubei Province, China.
2. Department of Blood Trausfusion, Suizhou Hospital Affiliated to Hubei Medical and Pharmaceutical College, Shuizou 441300, Hubei Province, China.
3. School of Basic Medicine, Hubei Medical and Pharmaceutical College, Shiyan 442000, Hubei Province, China.E-mail: denjunjob@163.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Adhesion;
Cell Adhesion Molecules;
Intercellular Adhesion Molecule-1;
Leukemia, B-Cell;
Mice;
Mice, Inbred NOD;
Mice, SCID;
Quercetin;
Vascular Cell Adhesion Molecule-1
- From:
Journal of Experimental Hematology
2018;26(6):1616-1620
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of Quercetin on cell cycle and adhesive molecules of NOD.SCID mice with acule B lymphocytic leuaemia(B-ALL).
METHODS:5×10 Nalm-6(B-ALL cell line) cells were injected into the tail vein of 48 NOD/SCID mice to establish the NOD/SCID mice with B-ALL. After 15 day, the NOD/SCID mice with B-ALL were randomly divided into 3 groups: salive group as control (injection with saline of 0.2 ml/mouse), cyclophos-phamid group (injection with cyclophosphamide of 100µg/kg) and quercetin group(injection with quercetin of 3 mg/kg). After treatment for 21 d, the perecntage of Nalm-6 cells in G1, G2, M and S phases was detected by flow cytonetry; the B lymphocytes Nalm-6 cells, neutrophils and WBC in while blood were counted before and after treatment; the expression of intercellalar. Adhesion molecole-1(FCAIU-1), vascular cell adhesion molecule-1(VCAM-1) and P-selectin was detected by double autibody soundwich method.
RESULTS:Compared with level before treatment, the expression of ICAM-1, VCAM-1 and P-selectin decreased after treatment with guercetin, The hemogram showed that the peripheral blood nentrophil level obviously increased, while the levels of B lymphocytes, Nalm-6 cells and WBC count decreased obviously after treatment with guercetin. The cell proliferatim rario in G0/G1 phase decreased, yet the cell proliferation ratio in S and G2/M phases increased after treatment with guercetin.
CONCLUSION:The guercetin can decrease the intercellular adhesion through inhibition of ICAM-1 expression, and arrests Nalm-6 cells in S and G2/M phases. The guercetin has obviously inhibitory effect on B-ALL cells.
